食品科学

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采后热空气处理对枇杷果实抗病性的诱导

王华东,王慧倩,郑 聪,王 静,郑永华*   

  1. 南京农业大学食品科技学院,江苏 南京 210095
  • 出版日期:2014-08-25 发布日期:2014-08-25
  • 通讯作者: 郑永华
  • 基金资助:

    国家自然科学基金面上项目(31172003);公益性行业(农业)科研专项(201003073)

Induction of Disease Resistances in Loquat Fruit by Postharvest Hot Air Treatment

WANG Hua-dong, WANG Hui-qian, ZHENG Cong, WANG Jing, ZHENG Yong-hua*   

  1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Online:2014-08-25 Published:2014-08-25
  • Contact: ZHENG Yong-hua

摘要:

研究热空气处理对枇杷果实腐烂和抗病相关酶活性及基因表达的影响。枇杷果实先用43 ℃热空气处理3 h,然后接种炭疽病菌,接种后的果实置于20 ℃条件下贮藏8 d。结果表明,热空气处理有效降低了枇杷果实的自然发病率,显著抑制了接种枇杷果实病斑直径的扩展,诱导了果实中几丁质酶、β-1,3-葡聚糖酶、苯丙氨酸解氨酶(PAL)和多酚氧化酶(PPO)等抗病相关酶活性的增强。热空气处理还可显著诱导接种枇杷果实PAL、PPO、过氧化物酶和热激蛋白基因的表达。同时,热空气处理对枇杷果实的硬度、可滴定酸和可溶性固形物含量等品质指标没有显著影响,但促进了类胡萝卜素的积累。以上结果表明,热空气处理可通过诱导抗病相关基因的表达和抗病相关酶活性的上升,提高枇杷果实的抗病能力。

关键词: 枇杷果实, 热空气处理, 刺伤接种, 诱导抗病性

Abstract:

The effects of hot air treatment on fruit decay and the activities and gene expression of defense-related enzymes in
postharvest loquat fruit were investigated. Freshly harvested loquat fruits were treated with hot air at 43 ℃ for 3 h, challenged
by being inoculated with Colletotrichum acutatum, and then stored at 20 ℃ for 8 d. The results showed that the fruit treated
with hot air had significantly lower natural disease incidence and smaller lesion diameter than the control fruit did. The hot air
treatment remarkably enhanced the activities of phenylalanine ammonia-lyase (PAL), chitinase, β-1,3-glucanase and polyphenol
oxidase(PPO). This hot air treatment also induced the expression of defense-related genes encoding PAL, PPO, peroxidase (POD)
and heat shock protein (HSP) 70. Meanwhile, this treatment did not significantly affect fruit firmness, titratable acidity and total
soluble solids, but enhanced the accumulation of carotenoids. These results suggest that hot air treatment can enhance disease
resistance of loquat fruit by inducing the activity and gene expression of defense-related enzymes.

Key words: loquat fruit, hot air treatment, challenging inoculation, induced disease resistance

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