食品科学

• 专题论述 • 上一篇    下一篇

环介导等温扩增技术在肠杆菌科致病菌检测中的研究进展

何晓华,顿玉慧,卢 力,李 可,刘 斌   

  1. 1.西北农林科技大学植物保护学院,陕西 杨凌 712100;2.浙江省检验检疫科学技术研究院,浙江 杭州 310016;
    3.义乌市检验检疫科学技术研究院,浙江 义乌 322000;4.西北农林科技大学食品科学与工程学院,陕西 杨凌 712100
  • 出版日期:2014-10-15 发布日期:2014-10-17

Recent Progress in Application of Loop-Mediated Isothermal Amplification Method for Detection of Enterobacteriaceae Pathogens

HE Xiao-hua, DUN Yu-hui, LU Li, LI Ke, LIU Bin   

  1. 1. College of Plant Protection, Northwest A&F University, Yangling 712100, China;
    2. Zhejiang Academy of Science & Technology for Inspection & Quarantine, Hangzhou 310016, China;
    3. Yiwu Academy of Science & Technology for Inspection & Quarantine, Yiwu 322000, China;
    4. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China
  • Online:2014-10-15 Published:2014-10-17

摘要:

环介导等温扩增(loop-mediated isothermal amplification,LAMP)技术是近年来新发展起来的一种恒温核酸扩增技术,该技术具有设备简单、检测速度快、灵敏度高、特异性强以及扩增效率高等优点,已被广泛地应用于食源性致病菌的检测研究。本文重点阐述近年来国内外学者应用LAMP技术在肠杆菌科中常见致病菌的检测研究进展。

关键词: 环介导等温扩增, 肠杆菌科, 致病菌, 检测

Abstract:

In recent years, loop-mediated isothermal amplification (LAMP) has emerged as a new technology for nucleic
acid amplification under constant temperature conditions, which is characterized by simple instrumental requirements,
rapid detection, high sensitivity, high specificity, and efficient amplification. This technology has been widely applied in
the detection of foodborne pathogens. This paper focuses on the recent progress in the application of LAMP assay in the
detection of common Enterobacteriaceae pathogens.

Key words: loop-mediated isothermal amplification (LAMP), Enterobacteriaceae, pathogens, detection

中图分类号: