食品科学

• 生物工程 • 上一篇    下一篇

γ-氨基丁酸产生菌的筛选及培养基优化

徐林敏,张 充,陆兆新,别小妹,赵海珍,吕凤霞*   

  1. 南京农业大学食品科技学院,江苏 南京 210095
  • 发布日期:2014-12-15

Screening of Gamma-Aminobutyric Acid-Producing Bacterium and Medium Optimization

XU Lin-min, ZHANG Chong, LU Zhao-xin, BIE Xiao-mei, ZHAO Hai-zhen, LÜ Feng-xia*   

  1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Published:2014-12-15

摘要:

采用纸层析法对南京地区多个不同场所采集的土样进行了菌种分离纯化,筛选到一株产γ-氨基丁酸(γ-aminobutyric acid,GABA)的菌株X-1,经形态特征与16S rDNA序列分析鉴定为巨大芽孢杆菌。通过单因素和正交试验对其发酵培养基进行优化,得到最佳培养基成分为葡萄糖30.0 g/L、蛋白胨30.0 g/L、K2HPO4 0.6 g/L、磷酸吡哆醇0.3 g/L、L-谷氨酸钠10.0 g/L、NaNO3 3.0 g/L、MgSO4·7H2O 0.5 g/L、FeSO4·7H2O 0.01 g/L。在此条件下,GABA浓度可达21.57 mmol/L,比优化前GABA浓度提高了3.83 倍。

关键词: &gamma, -氨基丁酸, 筛选, 培养基优化

Abstract:

A γ-aminobutyric acid (GABA)-producing strain X-1 was isolated from soil samples by paper chromatography.
Based on morphological characteristics, and 16S rDNA sequence and phylogenic analyses, X-1 was identified as Bacillus
megaterium. The culture medium of Bacillus megaterium X-1 was studied by single factor and orthogonal array designs.
The optimal medium components were 30.0 g/L glucose, 30.0 g/L peptone, 0.6 g/L K2HPO4, 0.3 g/L pyrodoxal-5-phosphate,
10.0 g/L monosodium glutamate, 3.0 g/L NaNO3, 0.5 g/L MgSO4·7H2O, and 0.01 g/L FeSO4·7H2O. Using the optimized medium,
the highest GABA yield of 21.57 mmol/L was obtained, representing a 4.83-fold increase over that before optimization.

Key words: γ-aminobutyric acid, screening, culture medium optimization

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