食品科学

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基于GC-MS指纹图谱和化学计量分析鉴别预包装纯葡萄汁及葡萄汁制品

李宝丽1,朱宇轩1,邓建玲1,谭 军1,刘 睿1,*,王 红2,李 涛2,周胜银2   

  1. 1.华中农业大学食品科学技术学院,环境食品学教育部重点实验室,湖北 武汉 430070;
    2.湖北省产品质量监督检验研究院,湖北 武汉 430061
  • 出版日期:2015-04-25 发布日期:2015-04-28
  • 通讯作者: 刘 睿
  • 基金资助:

    国家质检总局公益性行业科研专项(2012104002-4)

Discrimination of Prepackaged Pure Grape Juice and Its Commercial Products Based on Gas Chromatography-Mass Spectrometry Fingerprints and Chemometrics Methods

LI Baoli1, ZHU Yuxuan1, DENG Jianling1, TAN Jun1, LIU Rui1,*, WANG Hong2, LI Tao2, ZHOU Shengyin2   

  1. 1. Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology,
    Huazhong Agricultural University, Wuhan 430070, China;
    2. Hubei Research Institute of Products Quality Supervision and Inspection, Wuhan 430061, China
  • Online:2015-04-25 Published:2015-04-28
  • Contact: LIU Rui

摘要:

采用顶空固相微萃取-气相色谱-质谱联用技术研究预包装纯葡萄汁的香气成分,建立其香气成分的气相色谱-质谱指纹图谱,结合化学计量分析对3 个产地的混合果汁、纯葡萄汁、葡萄汁饮料共3 类预包装葡萄汁制品进行鉴别。结果表明:预包装纯葡萄汁中主要香气成分共38 种,所建立香气成分的气相色谱-质谱指纹图谱中共分析出15 个特征峰,其峰面积之和占总峰面积的95%以上。利用夹角余弦法和相关系数法对指纹图谱进行分析,发现气相色谱-质谱指纹图谱相似度与其品质呈正相关。所建立的香气成分指纹图谱结合化学计量分析能准确将预包装混合果汁、葡萄汁饮料和纯葡萄汁鉴别区分。

关键词: 预包装纯葡萄汁, 香气成分, 气相色谱-质谱指纹图谱, 化学计量分析, 质量评价

Abstract:

The aroma components of prepackaged pure grape juice were investigated and their gas chromatography-mass
spectrometry (GC-MS) fingerprints were established by solid phase micro-extraction-gas chromatography-mass spectrometry
(SPME-GC-MS) combined with similarity evaluation for the first time. By utilizing the fingerprinting, the qualities of
3 kinds of commercial grape juice from 3 regions were assessed. The results showed that 38 main aroma components were
identified in commercial grape juice, with a total of 15 peaks being detected as the characteristic peaks, and their areas
accounted for more than 95% of the total peak area. The GC-MS fingerprints were analyzed by angle cosine and correlation
coefficient. It was concluded that a positive correlation was found between the similarity of GC-MS chromatograms and the
quality of grape juice. Prepackaged mixed juice, grape juice beverage and pure grape juice could be accurately identified by
their aroma components fingerprints. The GC-MS fingerprints of prepackaged pure grape juice can provide a scientific basis
for the quality control and authenticity identification of grape juice.

Key words: prepackaged pure grape juice, aroma component, gas chromatography-mass spectrometry fingerprints, chemometrics methods, quality evaluation

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