食品科学

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常山胡柚果实不同部位有效成分含量及其 抗氧化活性比较

方博文,齐睿婷,张 英*   

  1. 浙江大学生物系统工程与食品科学学院,浙江省农产品加工技术研究重点实验室,浙江省食品加工技术与装备工程中心,
    浙江 杭州 310058
  • 出版日期:2015-05-25 发布日期:2015-05-08
  • 通讯作者: 张 英
  • 基金资助:

    浙江大学馥莉食品研究院基金资助项目(KY201303)

Active Components and Antioxidant Activities of Different Parts of Citrus paradise Fruit

FANG Bowen, QI Ruiting, ZHANG Ying*   

  1. Zhejiang R&D Center for Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing,
    College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China
  • Online:2015-05-25 Published:2015-05-08
  • Contact: ZHANG Ying

摘要:

将常山胡柚全果分为白皮层、油胞层、籽、果渣和果汁5 部分,除果汁外其余4 部分烘干后用70%乙醇溶液热回流提取制备不同部位的醇提物,分别测定醇提物总黄酮和总酚含量,并采用4 种自由基评价体系比较其抗氧化活性的差异。研究表明,常山胡柚不同部位醇提物中总黄酮含量的高低依次为:油胞层(3.78%)>白皮层(2.38%)>籽(1.67%)>果渣(0.77%),总酚含量最高也为油胞层(21.30%),最低为果渣(1.73%);油胞层清除1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基、O2-·和·OH的效果最佳,IC50值分别为0.37、0.24 mg/mL和1.31 mg/mL;而清除ABTS+·效果最好的则是白皮层(IC50值为0.015 mg/mL)。同时,测得胡柚果汁的总黄酮和总酚含量分别为0.021%和0.19%,清除DPPH自由基、ABTS+·、·OH和O2-·的IC50值分别为8.55、18.97、35.17 mg/mL和23.30 mg/mL。研究表明,胡柚果皮含有丰富的强抗氧化成分,且其质量约占全果的1/3,对其进行深入研究和综合开发是常山胡柚这一宝贵资源高值化利用过程中不可或缺的环节。

 

关键词: 常山胡柚, 果实不同部位, 醇提物, 果汁, 有效成分, 抗氧化活性

Abstract:

The fruit of Citrus paradisi was separated into albedo layer, oil gland layer, seed, pomace and juice. Active
substances were extracted from albedo layer, oil gland layer, seed and pomace with 70% aqueous ethanol by hot re?uxing.
The contents of total ?avonoids and phenolic substances in the resulting extracts were evaluated. Antioxidant activity was
tested in four systems. The results showed that the content of total ?avonoids in different fruit extracts of C. paradisi was
in the following order: oil gland layer (3.78%) > albedo layer (2.38%) > seed (1.67%) > pomace (0.77%). Similarly, the
content of total phenols was the highest in oil gland layer (21.30%) and the lowest in pomace (1.73%). The ethanol extract
of oil gland layer showed the highest antioxidant activity to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide
anion and hydroxyl radicals with IC50 of 0.37, 0.24 and 1.31 mg/mL, respectively. The ethanol extract of albedo layer
showed the highest ABTS+· scavenging activity with IC50 of 0.015 mg/mL. The contents of total flavonoids and total
phenols in the fresh juice were 0.021% and 0.19%, respectively. The IC50 values for DPPH free radical, ABTS+·, O2
-·, ·OH were 8.55, 18.97, 35.17 and 23.30 mg/mL, respectively. These results showed that oil gland layer and albedo layer had quite
good antioxidant activities, which were positively correlated with the contents of total ?avonoids and phenols. The pericarp
consisting of the two layers made up about one third of the total fruit weight, and was rich in potent antioxidant compounds.
To conclude, further study and development of C. paradisi fruit will have great practical signi?cance.

Key words: Citrus paradisi, different parts of whole fruit, alcohol extract, fresh juice, effective constituent; , antioxidant activity

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