食品科学

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噬菌体展示技术在食源性致病菌检测中的应用

胡雨欣,何 早,陈力力,刘 霞*   

  1. 湖南农业大学食品科学技术学院,食品科学与生物技术湖南省重点实验室,湖南 长沙 410128
  • 出版日期:2015-06-15 发布日期:2015-06-07

Phage Display Technology and Its Application in Detection of Foodborne Pathogens: a Review

HU Yuxin, HE Zao, CHEN Lili, LIU Xia*   

  1. Hunan Province Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology,
    Hunan Agricultural University, Changsha 410128, China
  • Online:2015-06-15 Published:2015-06-07

摘要:

噬菌体展示技术是20世纪80年代逐步建立并发展起来的一种分子生物学新技术,目前用于构建展示文库的噬菌体主要有丝状噬菌体、λ噬菌体、T4噬菌体和T7噬菌体。该技术在筛选噬菌体、单链抗体及多肽以建立食品安全检测体系方面,具有广阔的应用前景。本文在介绍噬菌体展示技术系统的基础上,对该技术在食源性致病菌检测中的应用进行综述。

关键词: 噬菌体展示技术, 食品致病菌, 检测

Abstract:

Phage display technology is a new molecular biological technology, which was gradually established and
developed since the 1980s. Currently there are mainly four types of phages used in the construction of phage display
libraries, including filamentous phage, λ phage, T4 and T7 phage. This technology has extensive applications in screening
specific phages, single-chain antibodies and peptides and establishing food safety detection system. In this article, the system
of phage display technology is described, and its application in the detection of foodborne pathogens is summarized.

Key words: phage display technique, foodborne pathogens, detection

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