对羟基苯甲醛等3 种天麻成分对灰树花胞外多糖生物合成的影响

doi:10.7506/spkx1002-6630-201607016

食品科学

对羟基苯甲醛等3 种天麻成分对灰树花胞外多糖生物合成的影响

吴彩云1，吴天祥1,2,*，朱俊杰1，刘昕1，赵群丽1

1.贵州大学酿酒与食品工程学院，贵州贵阳 550025；2.贵州大学食品科学工程技术研究中心，贵州贵阳 550025

出版日期:2016-04-15 发布日期:2016-04-13

Effect of p-Hydroxylbenzaldehyde and Other Main Ingredients of Rhizoma gastrodiae on the Biosynthesis of Exopolysaccharide by Grifola frondosa in Submerged Culture

WU Caiyun1, WU Tianxiang1,2,*, ZHU Junjie1, LIU Xin1, ZHAO Qunli1

1. School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China;
2. Research Center of Food Science and Engineering, Guizhou University, Guiyang 550025, China

Online:2016-04-15 Published:2016-04-13

摘要/Abstract

摘要：

在灰树花液体发酵体系中，分别添加不同量的天麻素、对羟基苯甲醇和对羟基苯甲醛3 种天麻成分，分析这3 种成分对灰树花菌体生长和胞外多糖（exopolysaccharide，EPS）合成的影响，采用苯酚-硫酸法测定胞外多糖含量。结果表明：适宜质量浓度的这3 种天麻成分均能促进灰树花菌体生长和胞外多糖的生物合成，其中对羟基苯甲醛添加量为0.15 g/L时效果最佳，并且略低于体积分数7%天麻醇提取物。动态分析0.15 g/L对羟基苯甲醛和7%天麻醇提取物对灰树花EPS合成促进作用的结果表明：在整个发酵过程中，二者促进灰树花EPS生物合成的效果基本一样，并且从发酵第7天开始，二者的EPS产量均显著高于空白组（P＜0.05）。采用高效液相色谱法动态测定发酵期间对羟基苯甲醛含量变化，结果表明：在发酵的第5天，对羟基苯甲醛基本被完全吸收，而此时灰树花EPS开始大量积累。因此，灰树花可利用对羟基苯甲醛来促进EPS的生物合成。

关键词: 对羟基苯甲醛, 天麻成分, 灰树花, 胞外多糖

Abstract:

To test the stimulatory effects of three ingredients of Rhizoma gastrodiae on the biosynthesis of exopolysaccharide
(EPS) by Grifola frondosa in submerged culture, gastrodin (GA), p-hydroxybenzyl alcohol (HA) and p-hydroxylbenzaldehyde
(HBA) were separately added at various concentrations into the culture medium. The EPS concentration in the culture
supernatant was determined by phenol-sulphuric acid assay. The results showed that all three additives had an effective
stimulatory effect on mycelial growth and EPS production. Among them, the addition of p-hydroxylbenzaldehyde at 0.15 g/L
exhibited the best effect, although slightly inferior to 7% (V/V) of the 75% ethanol extract of R. gastrodiae. Furthermore,
kinetic analysis suggested that the EPS production curves with 0.15 g/L p-hydroxybenzaldehyde and 7% (V/V) of the
R. gastrodiae extract over the entire fermentation process were substantially same, producing significantly more EPS than
the blank group from the 7th day onwards (P < 0.05). As determined by high performance liquid chromatography (HPLC),
dynamic changes in the concentration of p-hydroxylbenzaldehyde during the fermentation process showed that on the 5th
day, p-hydroxylbenzaldehyde was completely absorbed and EPS began to accumulate in large quantity. Therefore, Grifola
frondosa could utilize p-hydroxylbenzaldehyde to promote the biosynthesis of EPS.

Key words: p-hydroxylbenzaldehyde, ingredients of Rhizoma gastrodiae, Grifola frondosa, exopolysaccharide

中图分类号:

Q936

吴彩云，吴天祥，朱俊杰，刘昕，赵群丽. 对羟基苯甲醛等3 种天麻成分对灰树花胞外多糖生物合成的影响[J]. 食品科学, doi: 10.7506/spkx1002-6630-201607016.

WU Caiyun, WU Tianxiang, ZHU Junjie, LIU Xin, ZHAO Qunli. Effect of p-Hydroxylbenzaldehyde and Other Main Ingredients of Rhizoma gastrodiae on the Biosynthesis of Exopolysaccharide by Grifola frondosa in Submerged Culture[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201607016.

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对羟基苯甲醛等3 种天麻成分对灰树花胞外多糖生物合成的影响

Effect of p-Hydroxylbenzaldehyde and Other Main Ingredients of Rhizoma gastrodiae on the Biosynthesis of Exopolysaccharide by Grifola frondosa in Submerged Culture

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