关键词: 离子色谱, 脉冲安培, 配制酒, 氰化物

Abstract:

An ion chromatography with pulsed amperometric detection (IC-PAD) method was developed for the
determination of cyanide in mixed wines. The samples were diluted with ultrapure water and filtrated though a 0.22 μm MCM
membrane. The free cyanide was separated on a Thermo Fisher Dionex IonPac® AS16 anion exchange column kept at 30 ℃
using isocratic elution with a solution of 10 mmol/L sodium hydroxide as the mobile phase at a flow rate of 1.0 mL/min.
The eluates were detected with an Ag working electrode. The results revealed good linear responses of cyanide over the
range of 1–5 000 ng/mL) with a correlation coefficient (r) of 0.999 7, the limit of detection (LOD) was 0.1 mg/L at 25 μL
injection volume, and the average recovery was 102.3%. Compared to the spectrophotometric method described in the
Chinese national standard GB/T 5009.48—2003, this method is simple, rapid and more suitable for the determination of
cyanide in mixed wines with wide linear range, low LOD, high accuracy and good repeatability.

Key words: ion chromatography, pulsed amperometry, mixed wine, cyanide