食品科学

• 营养卫生 • 上一篇    下一篇

高通量测序研究益生菌对小鼠肠道菌群的影响

曲 巍1,2,张 智1,*,马建章3,*,刘 慧1,雅 男1   

  1. 1. 东北林业大学林学院,黑龙江 哈尔滨 150040;2. 黑龙江农业工程职业学院,黑龙江 哈尔滨 150088;
    3. 东北林业大学野生动物资源学院,黑龙江 哈尔滨 150040
  • 出版日期:2017-01-15 发布日期:2017-01-16

Effect of Probiotics on Gut Microbiota in Mice Evaluated by High-Throughput Sequencing

QU Wei1,2, ZHANG Zhi1,*, MA Jianzhang3,*, LIU Hui1, YA Nan1   

  1. 1. College of Forestry, Northeast Forestry University, Harbin 150040, China;
    2. Heilongjiang Agricultural Engineering Vocational College, Harbin 150088, China;
    3. College of Wildlife Resources, Northeast Forestry University, Harbin 150040, China
  • Online:2017-01-15 Published:2017-01-16

摘要: 采用高通量测序技术分析复合益生菌对昆明雄性小鼠肠道菌群的影响。利用0.9 mg/(g·d)青霉素钠灌胃建立肠道菌群失调小鼠模型。将50 只小鼠随机分为5 组:空白对照组、自然恢复组、复合菌液高、中、低3 个剂量组(复合益生菌浓度分别为109、108、107 CFU/mL )。0.2 mL/(d·10 g)连续灌胃14 d后,收集小鼠粪便,采用高通量测序技术对各组小鼠粪便菌群进行分析。结果表明:复合菌液高剂量组小鼠肠道菌群物种多样性最高(ACE值为22 101.63和Chao1值为13 791.40),自然恢复组最低;另外,小鼠肠道中有益菌与致病菌比例在各组之间差异显著,复合菌液各剂量组中有益菌群的比例均大于自然恢复组,而致病菌比例最高的为自然恢复组(56.36%),因此复合菌液对小鼠的肠道菌群失调具有调节作用。

关键词: 益生菌, 肠道菌群, 高通量测序

Abstract: The effect of mixed probiotic cultures on the intestinal microbiota of male Kunming mice was analyzed by
high-throughput sequencing in this investigation. An animal model of intestinal microflora imbalance was established
by intragastric administration of sodium penicillin to 40 mice, and these animals were randomly divided into natural
recovery group and three groups orally treated with mixed probiotic cultures at low, medium and high doses. Besides, a
blank group consisting of 10 normal mice was set up for control. After 14-d administration, feces were collected and the
intestinal microbiota were detected by high-throughput sequencing. The results showed that the high dose group had the
highest diversity of intestinal microbiota (ACE-index = 22 101.63; Chao1-index = 13 791.40) and the intestinal microbiota
in the natural recovery group exhibited the lowest species diversity. In addition, the results showed that the proportions of
beneficial and pathogenic bacteria in the mouse intestine differed significantly among the five groups; the proportions of
beneficial bacteria were higher in the three treatment groups than in the natural recovery group, and the highest proportion
of pathogenic bacteria (56.36%) was observed in the natural recovery group, suggesting that mixed probiotic cultures could
regulate intestinal microbiota imbalance in mice.

Key words: probiotic, intestinal microbiota, high-throughput sequencing

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