食品科学 ›› 2017, Vol. 38 ›› Issue (2): 227-232.doi: 10.7506/spkx1002-6630-201702036

• 工艺技术 • 上一篇    下一篇

壳聚糖没食子酸衍生物酶法催化条件优化及抗氧化活性和细胞毒性

吴 昊,王艺颖,甄天元,罗 丹,张 潇,杨绍兰,王成荣   

  1. 青岛农业大学食品科学与工程学院,山东 青岛 266109
  • 出版日期:2017-01-25 发布日期:2017-01-16
  • 基金资助:
    山东省现代农业(蔬菜)产业技术体系建设项目(SDAIT-05-21);山东省农业重大应用技术创新项目(SDNYCX-2015-ZD06-02);国家自然科学基金青年科学基金项目(31401549);山东省高等学校科技计划项目(J14LE11);青岛市科技计划项目(14-2-4-71-jch);青岛农业大学高层次人才科研基金项目(1207)

Optimization of Enzymatic Reaction between Chitosan and Gallic Acid for Enhanced Antioxidative Activity and Reduced Cytotoxicity

WU Hao, WANG Yiying, ZHEN Tianyuan, LUO Dan, ZHANG Xiao, YANG Shaolan, WANG Chengrong   

  1. College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China
  • Online:2017-01-25 Published:2017-01-16

摘要: 利用漆酶、辣根过氧化物酶在均相和非均相反应体系中催化壳聚糖与没食子酸反应,以增强壳聚糖的抗氧化性。研究酶种类、反应体系pH值、反应温度、反应时间、酶用量和底物的质量比等因素对产物接枝率的影响。利用单因素试验和正交试验确定的最适反应条件为反应pH 4.5、反应温度25 ℃、反应时间5 h、没食子酸与壳聚糖的质量比3∶1、漆酶用量4 U,此条件下衍生物的接枝率为65.2%。对衍生物分别进行体外抗氧化活性和细胞毒性检测,结果表明,在相同添加量的情况下,衍生物的抗氧化性显著高于未改性的壳聚糖,而且产物无细胞毒性。

关键词: 酶, 壳聚糖没食子酸衍生物, 抗氧化, 细胞毒性

Abstract: In this paper, chitosan (CTS) grafted copolymer with gallic acid (GA) was synthesized by laccase and horse radish peroxidase to enhance the antioxidant activity of chitosan. The effects of enzyme type and dosage, initial reaction pH, temperature, reaction time, and enzyme-to-substrate ratio on the grafting ratio were studied. The maximum grafting ratio (65.2%) was obtained when the reaction took place at 25 ℃ for 5 h in 100 mL of acetate buffer solution (100 mmol/L) at pH 4.5 with a mass ratio of GA to CTS of 3 in the presence of 4 U of laccase under constant stirring. At the same dosage, the CTSGA copolymer showed significantly higher antioxidant activity than chitosan and had no cytotoxicity.

Key words: enzyme, derivative from chitosan and gallic acid, antioxidant, cytotoxicity

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