食品科学 ›› 2017, Vol. 38 ›› Issue (21): 308-312.doi: 10.7506/spkx1002-6630-201721048

• 专题论述 • 上一篇    下一篇

克罗诺杆菌分型技术研究进展

费鹏,杨同香,姜亦超,陈俊亮,罗磊,任广跃,康怀彬,Stephen James FORSYTHE   

  1. (1.河南科技大学食品与生物工程学院,河南?洛阳 471023;2.长白山食品药品检验检测中心,吉林?白山 134500;3.诺丁汉特伦特大学病原体研究中心,英国?诺丁汉 NG11 8NS)
  • 出版日期:2017-11-15 发布日期:2017-11-01
  • 基金资助:
    河南科技大学博士科研启动基金项目(13480066);河南科技大学青年科学基金项目(2015QN038)

Progress in Typing Methods for Cronobacter spp.

FEI Peng, YANG Tongxiang, JIANG Yichao, CHEN Junliang, LUO Lei, REN Guangyue, KANG Huaibin, Stephen James FORSYTHE   

  1. (1. College of Food and Biological Engineering, Henan University of Science and Technology, Luoyang 471023, China; 2. Changbai Mountain Food and Drug Inspection Testing Center, Baishan 134500, China; 3. Pathogen Research Centre, Nottingham Trent University, Nottingham NG11 8NS, United Kingdom)
  • Online:2017-11-15 Published:2017-11-01

摘要: 克罗诺杆菌原名阪崎肠杆菌,是婴儿配方乳粉中主要的致病菌之一,能感染新生儿,并导致严重的坏死性小肠结肠炎、菌血症和脑膜炎等疾病,致死率高达40%~80%。本文综述了克罗诺杆菌常见的表型分型和分子分型方法,包括生化分型、基质辅助激光解吸电离飞行时间质谱分型、血清型分型、脉冲场凝胶电泳分型、多位点序列分型、随机多态性扩增DNA基因分型、核糖体分型、特殊基因分型和全基因组分型,以期增加人们对克罗诺杆菌分型方法的认识,为全面揭示克罗诺杆菌的表型特征和遗传多样性提供方法和依据。

关键词: 克罗诺杆菌, 表型分型, 分子分型, 分型技术

Abstract: Cronobacter spp., which was formerly defined as Enterobacter sakazakii, is one of the main pathogenic bacteria that easily contaminates powdered infant formula (PIF) and can infect newborns and cause severe necrotizing enterocolitis, bacteremia and meningitis, with a high mortality rate of 40%–80%. This article reviews the commonly used methods for the phenotypic and molecular typing of Cronobacter spp. including biotyping, matrix-assisted laser desorption ionization time of flight mass spectrometry, serological typing, pulsed field gel electrophoresis, multilocus sequence typing, random amplification of polymorphic DNA, ribotyping, special genotyping and whole-genome typing with the aim of providing a better understanding of these methods, which will offer a basis for revealing the phenotypic characteristics and genetic diversity of Cronobacter spp..

Key words: Cronobacter spp., phenotyping, molecular typing, typing methods

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