食品科学 ›› 2019, Vol. 40 ›› Issue (6): 289-295.doi: 10.7506/spkx1002-6630-20180312-147

• 工艺技术 • 上一篇    下一篇

昆布多糖不同提取工艺优化及其理化性质和抗肿瘤活性比较

李 莹,黄德春,陈贵堂*,艾宗华,蒋思琪,康译心,高仕婧   

  1. 中国药科大学工学院,江苏 南京 211198
  • 出版日期:2019-03-25 发布日期:2019-04-02
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2017YFD0400203);江苏省研究生科研与实践创新计划项目(KYCX17_0667);中国药科大学药学基地科研训练及科研能力提高项目(J1310032)

Polysaccharides from Laminaria japonica: Optimization of Different Extraction Processes and Comparison of Physicochemical Properties and Antitumor Activity

LI Ying, HUANG Dechun, CHEN Guitang*, AI Zonghua, JIANG Siqi, KANG Yixin, GAO Shijing   

  1. School of Engineering, China Pharmaceutical University, Nanjing 211198, China
  • Online:2019-03-25 Published:2019-04-02

摘要: 在单因素试验的基础上通过正交试验对昆布多糖的提取条件进行优化,并对比分析热水浸提法、超声辅助提取法和复合酶法3 种方法提取的昆布多糖的理化性质和抗肿瘤活性。结果表明,优化后昆布多糖的提取条件为,热水浸提法:提取温度85 ℃、提取时间5 h、料液比1∶80(g/mL),此条件下多糖平均提取率为5.97%;超声辅助提取法:超声功率500 W、超声时间10 min、料液比1∶90(g/mL),此条件下多糖平均提取率为5.81%;复合酶法:木瓜蛋白酶75 U/g、果胶酶15 000 U/g、纤维素酶108 000 U/g、pH 5、温度50 ℃、料液比1∶70(g/mL)、时间3 h,此条件下多糖平均提取率为14.29%。不同提取方法对昆布多糖的成分有显著影响,复合酶法提取的昆布多糖的溶解性显著优于水浴和超声提取。提取的昆布多糖中均具有少量蛋白质或多肽存在,并且具有三股螺旋结构。噻唑蓝(MTT)实验表明,不同提取方法得到的昆布多糖具有不同的抗肿瘤活性,其中超声辅助提取多糖对人肺癌细胞A549的抑制作用最强,而复合酶法所得多糖对人乳腺癌细胞MCF-7的抑制效果最好。

关键词: 昆布, 多糖, 提取方法, 理化性质, 抗癌活性

Abstract: Three extraction methods, including hot water extraction (HWE), ultrasonic-assisted extraction (UAE) and multiple enzymatic extraction (MEE), were used to extract polysaccharides from Laminaria japonica. Extraction conditions were optimized using one-factor-at-a-time and orthogonal array design methods, and the physicochemical properties and antitumor activity of the polysaccharides were comparatively analyzed. The results showed that the optimized HWE conditions were as follows: temperature 85 ℃, extraction time 5 h, and solid to solvent ratio 1:80 (g/mL); the optimized UAE conditions were ultrasonic power 500 W, extraction time, 10 min, and solid to solvent ratio 1:90 (g/mL); the optimized MEE conditions were papain 75 U/g, pectinase 15 000 U/g, cellulase 108 000 U/g, pH 5, temperature 50 ℃, solid to solvent ratio 1:70 (g/mL), and extraction time 3 h. Under the above conditions, the average yield of polysaccharides was 5.97%, 5.81% and 14.29%, respectively. There were significant effects of different extraction methods on the polysaccharide composition. The solubility of the polysaccharides obtained by MEE was significantly better than those obtained with HWE and UAE. All the polysaccharides contained a small amount of protein or polypeptide, and had three-strand helical structures. MTT assay showed that the three polysaccharides exhibited different antitumor activities, among which the polysaccharides obtained by UAE showed the strongest inhibitory effect on human lung cancer cell A549 while the polysaccharides extracted by CEE displayed the strongest inhibitory effect on human breast cancer cell line MCF-7.

Key words: Laminaria japonica, polysaccharide, extraction methods, physicochemical characteristics, antitumor activity

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