食品科学 ›› 2019, Vol. 40 ›› Issue (15): 154-161.doi: 10.7506/spkx1002-6630-20180817-175

• 营养卫生 • 上一篇    下一篇

具有肠道动力及肠道菌群调节功能乳杆菌的筛选及功能评价

沈旭丹,吉梦馨,翟齐啸,赵建新,张 灏,田丰伟,陈 卫   

  1. 江南大学食品学院,江苏 无锡 214122
  • 出版日期:2019-08-15 发布日期:2019-08-26
  • 基金资助:
    国家自然科学基金面上项目(31772090);国家食品科学与工程一流学科建设项目(JUFSTR20180102);江苏省食品安全与质量控制协同创新中心资助项目

Screening and Functionality Evaluation of Lactobacillus Able to Regulate Intestinal Motility and Flora

SHEN Xudan, JI Mengxin, ZHAI Qixiao, ZHAO Jianxin, ZHANG Hao, TIAN Fengwei, CHEN Wei   

  1. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
  • Online:2019-08-15 Published:2019-08-26

摘要: 便秘作为常见的胃肠道疾病,常伴有肠道动力及肠道菌群的异常。本研究通过测定45 株乳杆菌的体外生理特性,并筛选出3 株进行体内功能性评价,探讨乳杆菌对肠道动力以及肠道菌群的调节能力。结果表明:干酪乳杆菌LC9、发酵乳杆菌LF37和鼠李糖乳杆菌LR45的耐酸耐胆盐存活率高于10%,均能利用低聚果糖、低聚木糖、低聚半乳糖,且短链脂肪酸产量高于1.2 mmol/L。此外,3 株乳杆菌均能促进小鼠的小肠推进率,缩短首粒排黑便时间,增加粪便水分质量分数,加快肠道运动,同时能提高小鼠粪便菌群中有益菌的相对丰度,改善菌群结构。综上,这3 株菌对小鼠肠道功能有一定的调节作用,具有潜在的应用价值。

关键词: 乳杆菌, 肠道动力, 肠道菌群, 筛选

Abstract: Constipation is a common gastrointestinal disease frequently accompanied by abnormal intestinal motility and flora composition. The in vitro physiological characteristics of 45 strains of Lactobacillus were tested in the current study. Out of these, three strains were screened for in vivo functionality evaluation. Meanwhile, we evaluated the regulatory effect of Lactobacillus on intestinal motility and flora. The results indicated that survival rates of the selected strains, Lactobacillus casei LC9, Lactobacillus fermentum LF37 and Lactobacillus rhamnosus LR45, were all higher than 10% when exposed to acid and bile salt. All three strains were able to utilize oligofructose, xylo-oligosaccharide and galacto-oligosaccharide and the production of short chain fatty acids by each strain exceeded 1.2 mmol/L. Furthermore, a series of improved bowel functions were observed in the probiotic group, as manifested by the increased intestinal propulsion rate, the reduced first black stool defecation time, the augmented water content in feces, and the enhanced intestinal motility. The selected Lactobacillus could increase the relative abundance of beneficial bacteria in the mouse fecal flora and improve the structure of the fecal flora. The above results indicate that the three screened strains can regulate intestinal functions in mice, indicating their application potential.

Key words: Lactobacillus, intestinal motility, intestinal flora, screening

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