食品科学 ›› 2019, Vol. 40 ›› Issue (17): 79-84.doi: 10.7506/spkx1002-6630-20180821-226

• 基础研究 • 上一篇    下一篇

发光杆菌SN259发酵液中二苯乙烯类化合物的分离、鉴定及体外抗氧化活性

王保娟,汪钰,石丹姝,席雪冬,于志国   

  1. (1.沈阳农业大学植物保护学院,辽宁?沈阳 110866;2.辽宁省生物农药工程技术研究中心,辽宁?沈阳 110866)
  • 出版日期:2019-09-15 发布日期:2019-09-23
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2018YFD0800401)

Isolation, Identification and Antioxidant Activity of Stilbene Derivatives from Fermentation Broth of Photohabdus temperata SN259

WANG Baojuan, WANG Yu, SHI Danshu, XI Xuedong, YU Zhiguo   

  1. (1. College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China; 2. Engineering and Technological Research Center of Biopesticide for Liaoning Province, Shenyang 110866, China)
  • Online:2019-09-15 Published:2019-09-23

摘要: 为寻找高效天然的抗氧化剂,采用活性追踪法,利用固相萃取、柱层析、制备高效液相色谱等技术,对发光杆菌(Photohabdus temperata)SN259的发酵液进行分离纯化,得到9?个二烷基间苯二酚(dialkylresorcinols,DAR)衍生物类活性化合物,通过核磁共振和高效液相色谱-质谱综合解析和相关文献调研鉴定了所得活性化合物的结构,其中化合物1为新化合物,化合物2和3为首次分离纯化得到。对化合物1~9做抗氧化能力测试,并考察其对花生油氧化稳定性的影响,在2,2’-联氮-二(3-乙基-苯并噻唑-6-磺酸)阳离子自由基清除实验中,化合物1、8、9有较好的抗氧化能力,其自由基清除率达到50%时化合物的有效浓度分别为(4.95±0.11)、(6.17±0.09)、(4.55±0.07)μg/mL。

关键词: 抗氧化活性, 氧化稳定性, 亲脂性, 烷基间苯二酚

Abstract: In an effort to search for efficient antioxidants from natural resources, nine compounds were purified from the fermented broth of Photohabdus temperata SN259 using solid phase extraction, column chromatography, preparative high performance liquid chromatography by bioassay-guided fractionation. Their structures were identified by nuclear magnetic resonance (NMR) and high performance liquid chromatography-mass spectrometry through comparison with literature data. Compound 1 was a new one, and compounds 2 and 3 were isolated and purified for the first time. The antioxidant activity of compounds 1–9 was evaluated by their effect on the oxidation stability of peanut oil. Compounds 1, 8 and 9 showed 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical-scavenging activity with half maximal effective concentration values of (4.95 ± 0.11), (6.17 ± 0.09) and (4.55 ± 0.07) μg/mL, respectively.

Key words: antioxidant activity, oxidation stability, lipophilicity, dialkylresorcinols

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