食品科学 ›› 2019, Vol. 40 ›› Issue (19): 224-230.doi: 10.7506/spkx1002-6630-20181008-027

• 营养卫生 • 上一篇    下一篇

柑橘中苦味物质对HeLa细胞增殖与凋亡的作用机制

毕静莹,李华,王华   

  1. (1.西北农林科技大学葡萄酒学院,陕西 杨凌 712100;2.宁夏职业技术学院生物与制药技术系,宁夏 银川 750021)
  • 出版日期:2019-10-15 发布日期:2019-10-25
  • 基金资助:
    陕西省科技计划一般项目(2017NY-107);西北农林科技大学干部经费支持专项(Z11121850)

Mechanism of HeLa Cell Proliferation and Apoptosis Induced by Bitter Substances in Citrus

BI Jingying, LI Hua, WANG Hua   

  1. (1. College of Enology, Northwest A & F University, Yangling 712100, China; 2. Department of Biological and Pharmaceutical Technology, Ningxia Polytechnic, Yinchuan 750021, China)
  • Online:2019-10-15 Published:2019-10-25

摘要: 目的:以‘高台’蜜柚种子和白色海绵层为原料,提取柠檬苦素、诺米林和柚皮苷3 种苦味物质,分别探究它们对宫颈癌HeLa细胞增殖和凋亡的作用机制。方法:选择超声波辅助有机溶剂萃取和重结晶法,采用Acquity UPLC BEH C18色谱柱(2.1 mm×50 mm,1.7 μm),对3 种苦味物质提取、分离纯化和检测;在50 μmol/L的工作浓度下,以3 种苦味物质对HeLa细胞进行处理,利用细胞增殖检测、细胞划痕和逆转录-聚合酶链式反应实验进行抑癌作用的验证。结果:柠檬苦素类似物和柚皮苷的提取率分别为5.21 mg/g和7.72 mg/g;柠檬苦素、诺米林和柚皮苷的纯度分别为94.08%、93.91%和93.39%;抑癌活性实验证实了3 种苦味物质能抑制HeLa细胞增殖和迁移。

关键词: 柑橘, 柠檬苦素, 诺米林, 柚皮苷, HeLa细胞, 增殖与凋亡

Abstract: Purpose: To investigate how three bitter substances (limonin, nomilin and naringin) extracted from the seeds and peel of ‘Gaotai’ honey pomelo (Citrus grandis L.) impact the proliferation and apoptosis of HeLa cells. Methods: The substances were obtained and purified by ultrasonic-assisted organic solvent extraction and recrystallization and were detected by ultra-performance liquid chromatography (UPLC) using an Acquity UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm). Cell counting kit-8 (CCK-8) assay, cell scratch assay and reverse transcription-polymerase chain reaction (RT-PCR) were used to verify the anti-cancer effect of these substances at 50 μmol/L. Results: The extraction yields of limonoid and naringin were 5.21 and 7.72 mg/g, respectively. The purity of limonin, nomilin and naringin were up to 94.08%, 93.91% and 93.39%, respectively. The anti-cancer tests confirmed that the inhibition of HeLa cell growth by these bitter substances was closely related to the inhibition of cancer cell proliferation and migration.

Key words: citrus, limonin, nomilin, naringin, HeLa cells, proliferation and apoptosis

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