巴氏杀菌婴儿配方乳对大鼠T细胞亚群及其血液免疫相关因子的影响

doi:10.7506/spkx1002-6630-20181028-320

食品科学 ›› 2020, Vol. 41 ›› Issue (1): 157-162.doi: 10.7506/spkx1002-6630-20181028-320

巴氏杀菌婴儿配方乳对大鼠T细胞亚群及其血液免疫相关因子的影响

崔东影，席恩泽，徐境含，盛雪，李梦寒，刘洋，吴禹昊，许晓曦

（东北农业大学食品学院，黑龙江哈尔滨 150030）

出版日期:2020-01-15 发布日期:2020-01-19
基金资助:
“十二五”国家科技支撑计划项目（2013BAD18B00）

Effect of Pasteurized?Infant Formula on T Cell Subsets and Their Related Blood Immune Factors in Rats

CUI Dongying, XI Enze, XU Jinghan, SHENG Xue, LI Menghan, LIU Yang, WU Yuhao, XU Xiaoxi

(School of Food Science, Northeast Agriculture University, Harbin 150030, China)

Online:2020-01-15 Published:2020-01-19

摘要/Abstract

摘要： 目前市场上的婴儿配方食品主要以婴儿配方乳粉为主，本实验主要研究巴氏杀菌婴儿配方乳对大鼠T细胞亚群及血清免疫因子的影响。将健康SD雄性大鼠分成4 组：基础日粮组、市售常温婴儿配方乳I组、市售常温婴儿配方乳II组、巴氏杀菌婴儿配方乳组，以大鼠体质量，血清中白细胞介素（interleukin，IL）-2、IL-4、干扰素-γ（interferon-γ，IFN-γ）、肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）、溶菌酶水平以及全血中CD3＋、CD4＋、CD8＋淋巴细胞相对含量，CD4＋/CD8＋为测定指标，对比探讨几种婴儿配方乳对SD大鼠免疫调节作用的影响。巴氏杀菌婴儿配方乳和市售常温婴儿配方乳组大鼠体质量显著高于基础日粮组（P＜0.05）。巴氏杀菌婴儿配方乳组大鼠血清中IL-2、TNF-α和溶菌酶水平显著高于其他两组（P＜0.05），IFN-γ水平显著高于基础日粮组（P＜0.05），但与市售婴儿配方乳组差异不显著（P＞0.05）；3 组大鼠血清中IL-4水平都不同程度降低，但市售常温婴儿配方乳和巴氏杀菌婴儿配方乳组显著高于基础日粮组（P＜0.05）。巴氏杀菌婴儿配方乳组大鼠全血中CD3＋、CD4＋、CD8＋淋巴细胞相对含量分别为（34.90±1.15）%、（23.93±2.11）%、（9.17±0.42）%。结果表明，与市售常温婴儿配方乳比较，巴氏杀菌婴儿配方乳会促进SD大鼠生长及淋巴细胞的增生分化，增强体液免疫和细胞免疫功能，并辅助机体免疫调节。

关键词: 巴氏杀菌婴儿配方乳, T细胞亚群, 血液免疫因子

Abstract: Infant formula is one of the major infant formula products currently available on the market. In this experimental study, the effect of pasteurized infant formula on T cell subsets and serum immune factors in rats was explored. Healthy SD male rats were divided into 4 ?dietary treatment?groups including basal diet, commercial normal temperature infant formulas I and II, and pasteurized infant formula. Body mass, the serum levels of interleukin (IL)-2, IL-4, interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α) and lysozyme, and the relative contents of CD3+, CD4+ and CD8+ lymphocytes and CD4+/CD8+ ratio in whole blood were measured. The body mass of rats administered with the three infant formulas was significantly higher than that of rats in the basal diet group (P < 0.05). The serum levels of IL-2, TNF-α and lysozyme in the pasteurized infant formula group were significantly higher than those in the other infant formula groups (P < 0.05), and the IFN-γ level was significantly higher than that in the basal diet group (P < 0.05) but was not significantly different from that in the other infant formula groups. The serum levels of IL-4 in the three experimental groups decreased to different extents with administration time, but they were significantly higher than that in the basal diet group (P < 0.05). The relative contents of CD3+, CD4+ and CD8+ lymphocytes in the whole blood of rats given pasteurized infant formula were (34.90 ± 1.15)%, (23.93 ± 2.11)%, and (9.17 ± 0.42)%, respectively. These results showed that pasteurized infant formula promoted the growth of SD rats and the proliferation and differentiation of lymphocytes, enhanced humoral immunity and cellular immune function, and improved immune regulation in rats when compared with commercial infant formula.

Key words: pasteurized infant formula, T cell subsets, blood immune factors

中图分类号:

TS252.1

崔东影，席恩泽，徐境含，盛雪，李梦寒，刘洋，吴禹昊，许晓曦. 巴氏杀菌婴儿配方乳对大鼠T细胞亚群及其血液免疫相关因子的影响[J]. 食品科学, 2020, 41(1): 157-162.

CUI Dongying, XI Enze, XU Jinghan, SHENG Xue, LI Menghan, LIU Yang, WU Yuhao, XU Xiaoxi. Effect of Pasteurized?Infant Formula on T Cell Subsets and Their Related Blood Immune Factors in Rats[J]. FOOD SCIENCE, 2020, 41(1): 157-162.

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巴氏杀菌婴儿配方乳对大鼠T细胞亚群及其血液免疫相关因子的影响

Effect of Pasteurized?Infant Formula on T Cell Subsets and Their Related Blood Immune Factors in Rats

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