食品科学 ›› 2020, Vol. 41 ›› Issue (1): 203-208.doi: 10.7506/spkx1002-6630-20181102-023

• 营养卫生 • 上一篇    下一篇

西番莲种籽中总黄酮的体内抗氧化活性及其成分分析

李焱,黄苇,卢明剑,胡志敏,吕秋洁   

  1. (1.华南农业大学食品学院,广东 广州 510642;2.华南农业大学工程学院,广东 广州 510642)
  • 出版日期:2020-01-15 发布日期:2020-01-19
  • 基金资助:
    亚热带农产品梯级加工共性关键技术研发项目(2018LM2154)

In Vivo Antioxidant Capacity and Flavonoid Composition of the Seeds of Passiflora edulis Sims

LI Yan, HUANG Wei, LU Mingjian, HU Zhimin, Lü Qiujie   

  1. (1. College of Food Science, South China Agricultural University, Guangzhou 510642, China; 2. College of Engineering, South China Agricultural University, Guangzhou 510642, China)
  • Online:2020-01-15 Published:2020-01-19

摘要: 以西番莲种籽中的总黄酮为研究对象,通过构建D-半乳糖氧化损伤动物实验模型,测定模型小鼠肝、肾组织的总超氧化物歧化酶(total superoxide dismutase,T-SOD)、过氧化氢酶(catalase,CAT)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力以及丙二醛(malondialdehyde,MDA)、蛋白羰基含量等指标,研究其体内抗氧化活性。结果表明:种籽中的总黄酮可显著提高氧化损伤模型小鼠肝、肾组织的T-SOD及CAT、GSH-Px活力(P<0.05),显著降低MDA、蛋白质羰基含量(P<0.05),具有良好的体内抗氧化活性。以甲醇-0.25%甲酸溶液为流动相进行梯度洗脱,采用液相色谱-电喷雾四极杆飞行时间质谱对西番莲种籽中黄酮成分进行分析。根据准分子离子和碎片离子信息,结合参考文献,推测出4?种西番莲种籽黄酮成分分别为2-(3,4-二羟基苯基)-5,7-二羟基-6-异戊基-4H-苯并吡喃-4-酮、2-(4-羟基苯基)-3,6-二甲氧基-8,8-二甲基-4H,8H-吡喃并[2,3-f]苯并吡喃-4酮、5-羟基-2-(4-羟基-3-甲氧苯基)-3-甲氧基-7-((3-甲基-2-丁烯-1-基)氧代)-4H-苯并吡喃-4-酮、7-((4-甲氧苄基)氧代)-3-(2-甲氧苯基)-2-羧酸乙酯-4H-苯并吡喃-4-酮。

关键词: 西番莲种籽, 黄酮, 体内抗氧化活性, 液相色谱-电喷雾四极杆飞行时间质谱

Abstract: The in vivo antioxidant capacity of total flavonoids from the seeds of Passiflora edulis Sims were studied by measuring total superoxide dismutase (T-SOD) activity, catalase (CAT) activity, glutathione peroxidase (GSH-Px) activity, malondialdehyde (MDA) content and the content of protein carbonyl in liver and kidney tissues of a mouse model of D-galactose-induced oxidative damage after oral administration with the total flavonoids. Results showed that the total flavonoids could significantly increase T-SOD, CAT and GSH-Px activities (P < 0.05), and reduce the contents of MDA and protein carbonyl in mouse liver and kidney tissues (P < 0.05), indicating good antioxidant capacity in vivo. Based on gradient elution using methanol (A) and 0.25% formic acid (B) as mobile phase, the flavonoids were analyzed by liquid chromatography coupled with electrospray ionization-quadrupole-time of flight mass spectrometry (LC-ESI-Q-TOF-MS). According to the accurate mass spectral information obtained from excimer and fragment ions as well as literature data, 4 constituents were tentatively presumed, including 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-6-isopentyl-4H-chromen-4-one, 2-(4-hydroxyphenyl)-3,6-dimethoxy-8,8-dimethyl-4H,8H-pyrano[2,3-f]chromen-4-one, 5-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-3-methoxy-7-((3-methylbut-2-en-1-yl)oxy)-4H-chromen-4-one, and 7-((4-methoxybenzyl)oxy)-3-(2-methoxyphenyl)-4-oxo-4H-chromene-2-carboxylate.

Key words: seeds of Passiflora edulis Sims, flavonoids, in vivo antioxidant activity, liquid chromatography coupled with electrospray ionization-quadrupole-time of flight mass spectrometry

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