鞣花酸微球的制备及其对前脂肪细胞生长和成脂分化的影响

doi:10.7506/spkx1002-6630-201817017

食品科学 ›› 2018, Vol. 39 ›› Issue (17): 104-108.doi: 10.7506/spkx1002-6630-201817017

鞣花酸微球的制备及其对前脂肪细胞生长和成脂分化的影响

马晶，康美玲，田忠景，张营霞，丁诚实*，张立华*

枣庄学院生命科学学院，山东枣庄 277160

出版日期:2018-09-15 发布日期:2018-09-18
基金资助:
国家自然科学基金青年科学基金项目（81703190）；山东省重点研发项目（GG201703210015）；山东省自然科学基金项目（ZR2017LH058）；枣庄学院博士基金项目（2016BS01）

Preparation of Ellagic Acid Microspheres and Their Effects on Preadipocyte Growth and Adipogenic Differentiation

MA Jing, KANG Meiling, TIAN Zhongjing, ZHANG Yingxia, DING Chengshi*, ZHANG Lihua*

College of Life Sciences, Zaozhuang University, Zaozhuang 277160, China

Online:2018-09-15 Published:2018-09-18

摘要/Abstract

摘要： 目的：采用海藻酸钠/壳聚糖对石榴皮鞣花酸进行包裹，制备成鞣花酸微球，并观察其对前脂肪细胞的生长及成脂分化的影响。方法：利用扫描电子显微镜、傅里叶变换红外光谱仪和释放率分析仪鉴定制备的鞣花酸微球；噻唑蓝法检测不同质量浓度的鞣花酸微球在6、12、24 h和36 h时对前脂肪细胞生长的影响，苏木精-伊红染色观察细胞形态及数量的变化；油红O染色观察鞣花酸微球对前脂肪细胞成脂分化的影响，异丙醇萃取测定脂肪生成量。结果：成功制备了海藻酸钠/壳聚糖包裹的鞣花酸微球，0.1、0.3 g/L和0.5 g/L的鞣花酸微球能够抑制前脂肪细胞的生长及成脂分化，且具有剂量-效应关系，对脂滴生成的抑制率可达80%。结论：以海藻酸钠/壳聚糖作为石榴皮鞣花酸的包裹材料制备鞣花酸微球是可行的，鞣花酸微球具备潜在的保健价值。

关键词: 鞣花酸微球, 制备, 鉴定, 前脂肪细胞生长, 成脂分化

Abstract: Objective: In this study, sodium alginate and chitosan as a coating were chosen for the preparation of ellagic acid microspheres, and the effect of the microspheres on the growth and adipogenic differentiation of preadipocytes was evaluated. Methods: Scanning electron microscopy, Fourier transform infrared spectra and release rate analysis were used to identify the formation of ellagic acid microspheres. Methylthiazolyldiphenyl-tetrazolium bromide method was used to detect the effect of different concentrations of ellagic acid microspheres on preadipocyte growth at 6, 12, 24 and 36 h, and the cell morphology and quantity were observed by hematoxylin-eosin staining. Oil red O staining was used to observe the effect of ellagic acid microspheres on adipogenic differentiation of preadipocyte cells, and lipogenesis was detected by isopropanol extraction. Results: Ellagic acid microspheres were successfully prepared. Ellagic acid microspheres at 0.1, 0.3 and 0.5 g/L could inhibit the growth and adipogenic differentiation of preadipocytes in a dose-dependent manner, and showed 80% inhibition on lipid droplet formation. Conclusion: It is feasible to prepare ellagic acid microspheres with sodium alginate and chitosan, and ellagic acid microspheres have potential therapeutic value for obesity.

Key words: ellagic acid microspheres, preparation, identification, preadipocyte growth, adipogenic differentiation

中图分类号:

TS202.3

马晶，康美玲，田忠景，张营霞，丁诚实，张立华. 鞣花酸微球的制备及其对前脂肪细胞生长和成脂分化的影响[J]. 食品科学, 2018, 39(17): 104-108.

MA Jing, KANG Meiling, TIAN Zhongjing, ZHANG Yingxia, DING Chengshi, ZHANG Lihua. Preparation of Ellagic Acid Microspheres and Their Effects on Preadipocyte Growth and Adipogenic Differentiation[J]. FOOD SCIENCE, 2018, 39(17): 104-108.

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鞣花酸微球的制备及其对前脂肪细胞生长和成脂分化的影响

Preparation of Ellagic Acid Microspheres and Their Effects on Preadipocyte Growth and Adipogenic Differentiation

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