食品科学 ›› 2018, Vol. 39 ›› Issue (22): 173-178.doi: 10.7506/spkx1002-6630-201822027

• 生物工程 • 上一篇    下一篇

虾酱中间产物细菌群落结构及多样性

戴玲瑛1,齐曼婷2,王立梅3,齐斌3,*   

  1. (1.江南大学食品学院,江苏?无锡 214122;2.谱尼测试集团江苏有限公司,江苏?苏州 215000;3.常熟理工学院生物与食品工程学院,苏州市食品生物技术重点实验室,发酵工程技术研究中心,江苏?常熟 215500)
  • 出版日期:2018-11-25 发布日期:2018-11-21
  • 基金资助:
    江苏省科技厅重点研发计划项目(BE2017316;BE2017326)

Bacterial Community Structure and Diversity in Unsterilized Shrimp Sauce

DAI Lingying1, QI Manting2, WANG Limei3, QI Bin3,*   

  1. (1. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China;2. Pony Testing International Group, Suzhou 215000, China;3. Key Laboratory of Food and Biotechnology of Suzhou, Fermentation Engineering Technology Research Center, College of Biological and Food Engineering, Changshu Institute of Technology, Changshu 215500, China)
  • Online:2018-11-25 Published:2018-11-21

摘要: 为更全面地探究虾酱中微生物的多样性,运用高通量测序技术,对虾酱中细菌16S?rDNA?V3-V4区测序,从而对虾酱中的细菌群落组成和多样性进行分析。研究共获得61?107?条序列,4?358?个操作分类单元,多样性分析表明虾酱中具有高度的微生物群落多样性。虾酱微生物群落组成分析表明虾酱中变形菌门占92.02%,γ-变形菌纲占90.58%,弧菌目占85.91%,弧菌科占85.91%,发光杆菌属占71.94%。研究结果加深了对虾酱中微生物群落组成和多样性的认识,为保证虾酱的质量与安全,提高虾酱的品质,优化虾酱的生产工艺提供理论依据。

关键词: 虾酱, 微生物多样性, 高通量测序技术

Abstract: This study aimed to fully understand the microbial diversity in shrimp sauce. The V3-V4 region of the bacterial 16S rDNA gene from shrimp sauce was sequenced by high-throughput sequencing, and the composition and diversity of the bacterial communities were analyzed. A total of 61 107 effective sequences and 4 358 operational taxonomic units (OTUs) were obtained. The result of diversity analysis showed that the semi-finished product had a high microbial community diversity. The analysis of bacterial composition indicated that Proteobacteria, Gammaproteobacteria, Vibrionales, Vibrionaceae and Photobacterium accounted for 92.02%, 90.58%, 85.91%, 85.91% and 71.94%, respectively. This research gives a better understanding of the microbial communities in shrimp sauce, which can provide a theoretical basis for ensuring the quality and safety, improving the quality and optimizing the production of shrimp sauce.

Key words: shrimp sauce, microbial diversity, high-throughput sequencing

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