不同来源红缘拟层孔菌粗多糖的抗氧化活性

doi:10.7506/spkx1002-6630-20190329-381

食品科学 ›› 2019, Vol. 40 ›› Issue (19): 60-68.doi: 10.7506/spkx1002-6630-20190329-381

不同来源红缘拟层孔菌粗多糖的抗氧化活性

聂琳然，郝利民,，王滔滔，刘阳，张黎明，鲁吉珂，康彩彩，崔燕，韩培培，贾士儒

（1.天津科技大学工业发酵微生物教育部重点实验室，天津 300457；2.军事科学院军需工程技术研究所，北京 100010；3.郑州大学生命科学学院，河南郑州 450001）

出版日期:2019-10-15 发布日期:2019-10-25
基金资助:
“十三五”国家重点研发计划重点专项（2018YFD0400204）；全军重点科研项目（BX115C007；AX110C002）

Antioxidant Activity of Crude Polysaccharides from Fomitopsis pinicola from Different Geographical Origins

NIE Linran, HAO Limin,, WANG Taotao, LIU Yang, ZHANG Liming, LU Jike, KANG Caicai, CUI Yan, HAN Peipei, JIA Shiru

(1. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology,Tianjin 300457, China; 2. Quartermaster Engineering Technology Institute, Academy of Military Sciences PLA China, Beijing 100010, China; 3. School of Life Sciences, Zhengzhou University, Zhengzhou 450001, China)

Online:2019-10-15 Published:2019-10-25

摘要/Abstract

摘要： 采用1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl，DPPH）自由基、2,2’-联氮基双(3-乙基苯并噻唑啉-6-磺酸)（2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)，ABTS）阳离子自由基、羟自由基清除实验和铁氰化钾还原法，对3 种不同产地（吉林、黑龙江、云南）红缘拟层孔菌子实体（Fomitopsis pinicola fruiting body，分别记为JFPF、HFPF、YFPF）、红缘拟层孔菌菌丝体（Fomitopsis pinicola mycelium，FPM）及其发酵液（Fomitopsis pinicola fermentation broth，FPFB）粗多糖的体外抗氧化活性进行对比研究；并采用紫外线和H2O2氧化损伤酵母细胞保护实验模型，评价上述5 种粗多糖对氧化损伤酵母细胞的保护作用。结果表明：这5 种红缘拟层孔菌粗多糖均表现出不同程度的体外抗氧化活性，并均能明显提高氧化损伤酵母细胞的存活率，且呈一定的剂量依赖效应；其中，YFPF粗多糖对DPPH自由基、ABTS阳离子自由基、羟自由基的清除能力及还原能力在5 种粗多糖中较强；在质量浓度为5 mg/mL时，其对3 种自由基的清除率分别为78.78%、99.24%、64.38%，还原能力为0.84。FPM粗多糖对氧化损伤酵母细胞保护作用最强，可明显提高氧化损伤酵母细胞的存活率，当其质量浓度为20 mg/mL时，紫外和H2O2氧化损伤酵母细胞的存活率分别为75.48%、48.38%。因此，不同来源红缘拟层孔菌粗多糖的抗氧化活性存在差异，这为红缘拟层孔菌的开发利用提供理论依据。

关键词: 红缘拟层孔菌, 粗多糖, 氧化损伤, 抗氧化活性

Abstract: The present work was undertaken to investigate the antioxidant activity of crude polysaccharides extracted from Fomitopsis pinicola fruiting bodies from different geographical origins: Jilin, Heilongjiang and Yunnan provinces (designated as JFPF, HFPF and YFPF, respectively), Fomitopsis pinicola mycelium (FPM) and its fermentation broth (FPFB). The antioxidant activity was evaluated by determining the scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and hydroxyl radicals and K3Fe(CN)6 reducing power, followed by the evaluation of the protective effects against ultraviolet (UV) radiation and H2O2-induced?oxidative damage in yeast cells. The results showed that the five crude polysaccharides had different antioxidant activities and significantly increased the survival rate of yeast cells under oxidative damage in a dose-dependent manner. The scavenging capacity against DPPH, ABTS and hydroxyl radicals, and the reducing power of YFPF polysaccharides were relatively stronger among these polysaccharides. At 5 mg/mL, the scavenging rates of DPPH, ABTS and hydroxyl radicals were 78.78%, 99.24% and 64.38%, respectively, and the reducing power was 0.84. In addition, the protective effect of FPM polysaccharides against oxidative damage was the strongest, as evidenced by significantly improved survival rate of yeast cells. At 20 mg/mL, the survival rates of yeast cells under UV and H2O2-induced oxidative damage were 75.48% and 48.38%, respectively. This study demonstrated that the antioxidant activity of crude polysaccharides from F. pinicola varied with geographical origin, which will provide a theoretical basis for the exploitation and utilization of F. pinicola.

Key words: Fomitopsis pinicola, crude polysaccharides, oxidative damage, antioxidant activity

中图分类号:

TS201.1

聂琳然，郝利民,，王滔滔，刘阳，张黎明，鲁吉珂，康彩彩，崔燕，韩培培，贾士儒. 不同来源红缘拟层孔菌粗多糖的抗氧化活性[J]. 食品科学, 2019, 40(19): 60-68.

NIE Linran, HAO Limin,, WANG Taotao, LIU Yang, ZHANG Liming, LU Jike, KANG Caicai, CUI Yan, HAN Peipei, JIA Shiru. Antioxidant Activity of Crude Polysaccharides from Fomitopsis pinicola from Different Geographical Origins[J]. FOOD SCIENCE, 2019, 40(19): 60-68.

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不同来源红缘拟层孔菌粗多糖的抗氧化活性

Antioxidant Activity of Crude Polysaccharides from Fomitopsis pinicola from Different Geographical Origins

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