关键词: 多糖, 表征, 抗氧化活性, 毒性

Abstract: In the present study, polysaccharides were obtained from the leaves of Rubus suavissimus S. Lee by water extraction followed by alcohol precipitation. The polysaccharide content and monosaccharide composition were determined. The antioxidant activity in vitro of the polysaccharides was evaluated by reducing power, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and hydroxyl radical (·OH) scavenging activity. Meanwhile, toxicity studies in vivo were conducted in the zebrafish model. The results showed that the yield of polysaccharides extracted from Rubus suavissimus S. Lee was 0.6%. The total sugar content of the polysaccharides was 70.2%, and the monosaccharide composition mainly included mannose, rhamnose, glucose, galactose, arabinose and fucose at a molar ratio of 1.17:21.79:3.54:1.00:1.33:2.86. The polysaccharides had antioxidant activity in vitro in terms of reducing power, DPPH radical scavenging activity and ·OH radical scavenging activity in a dose-dependent manner. In the in vivo toxicity studies, the polysaccharides did not exhibit teratogenic or lethal effects on zebrafish embryos and juveniles and could be safe and low toxic to some extent.

Key words: polysaccharides, characterization, antioxidant activity, toxicity