食品科学 ›› 2020, Vol. 41 ›› Issue (2): 80-86.doi: 10.7506/spkx1002-6630-20190630-421

• 生物工程 • 上一篇    下一篇

不同载体固定化蒙氏肠球菌发酵蛋壳制备乳酸钙

赵静丽,刘远远,马美湖   

  1. (华中农业大学食品科学技术学院,国家蛋品加工技术研发中心,湖北 武汉 430070)
  • 出版日期:2020-01-25 发布日期:2020-01-19
  • 基金资助:
    国家自然科学基金面上项目(31571784)

Production of Calcium Lactate from Eggshell Fermentation by Enterococcus mundtii Cells Immobilized on Different Carriers

ZHAO Jingli, LIU Yuanyuan, MA Meihu   

  1. (National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China)
  • Online:2020-01-25 Published:2020-01-19

摘要: 针对我国每年废弃的大量蛋壳,采用海藻酸钠、海藻酸钠-聚乙烯醇和海藻酸钠-活性炭(sodium alginate-activated carbon,SA-C)3 种载体固定化蒙氏肠球菌发酵蛋壳废物生产乳酸钙,考察并比较其机械强度、传质系数、产乳酸钙性能及最佳包埋载体的性质。结果表明,SA-C载体是最适的包埋载体,其乳酸钙产量最高达到101.02 g/L,蛋壳粉转化率为82.43%;在重复发酵18 次后仍保持高产率,且在重复发酵10 次有缩短发酵周期的趋势。发酵液中蛋壳废物的存在有效维持了固定化细胞的机械稳定性,与游离细胞相比,其细胞操作稳定性、温度、pH值及贮存稳定性在一定范围内都有明显的提高。

关键词: 海藻酸钠, 海藻酸钠-聚乙烯醇, 海藻酸钠-活性炭, 蛋壳废物, 乳酸钙

Abstract: A large amount of eggshell is produced and discarded in China annually. In order to utilize this waste, Enterococcus mundtii was immobilized on sodium alginate (SA), sodium alginate-polyvinyl alcohol (SA-PVA) or sodium alginate-activated carbon (SA-C) and then used to convert eggshell to calcium lactate. The three immobilized cells were compared in their mechanical strength, mass transfer and calcium lactate production ability and the best one was characterized. The results showed that SA-C was found to be the optimum immobilization carrier that provided the maximum yield of calcium lactate of 101.02 g/L with a conversion rate of 82.43%. The yield of calcium lactate remained high after repeated fermentation for 18 times, and the fermentation cycle tended to be shortened by repeated use. The presence of residual eggshell in the fermentation broth effectively maintained the mechanical stability of the immobilized cells. Compared with the free cells, the operating stability as well as temperature, pH and storage stability of the immobilized cells were significantly improved in a certain range.

Key words: sodium alginate, sodium alginate-polyvinyl alcohol, sodium alginate-activated carbon, eggshell waste, calcium lactate

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