食品科学 ›› 2020, Vol. 41 ›› Issue (16): 21-28.doi: 10.7506/spkx1002-6630-20190715-199

• 食品化学 • 上一篇    

多酚对热处理甜瓜汁挥发性关键异味组分的抑制作用

孙钰清,孔凡玉,吴继红,庞雪莉   

  1. (1.中国农业科学院烟草研究所,山东 青岛 266101;2.中国农业大学食品科学与营养工程学院,北京 100048)
  • 发布日期:2020-08-19
  • 基金资助:
    家自然科学基金青年科学基金项目(31601483);国家特色农产品质量安全风险评估专项(GJFP201801003); 中国农业科学院科技创新工程项目(ASTIP-TRIC-06)

Inhibitory Effect of Polyphenols on Key Off-odors in Thermally Processed Muskmelon Juice

SUN Yuqing, KONG Fanyu, WU Jihong, PANG Xueli   

  1. (1. Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China; 2. College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100048, China)
  • Published:2020-08-19

摘要: 为探究不同种类多酚对热处理甜瓜汁挥发性异味的抑制效果,通过感官评价结合Friedman检验和多重比较统计方法,比较评价不同多酚的异味抑制作用;采用顶空固相微萃取结合气相色谱-质谱联用对不同多酚添加前后的甜瓜汁异味组分进行定性定量分析,并结合正交偏最小二乘-判别分析法分析不同多酚对异味组分的作用特征。研究发现,感官评价和不同多酚添加组中受抑制异味种类和异味抑制率的仪器分析结果一致,表明0.1 mg/mL的儿茶素、染料木黄酮、黄豆苷元、海藻多酚及邻苯三酚处理对热处理甜瓜汁的异味生成均有不同程度的抑制作用;其中儿茶素的抑制效果最佳,其次是海藻多酚、黄豆苷元、染料木黄酮和邻苯三酚,且儿茶素、海藻多酚、黄豆苷元均具有显著的异味抑制效果(P<0.05);儿茶素和黄豆苷元可作为抑制异味的最佳组合。本研究结果可为以甜瓜为代表的热敏性果蔬的热异味问题的攻克及深加工产品开发提供理论借鉴。

关键词: 甜瓜;热处理;挥发性异味;多酚化合物;异味抑制

Abstract: This study investigated and compared the inhibitory effect of various polyphenols on the formation of off-odors in thermally processed muskmelon juice using sensory evaluation combined with the Friedman test and multiple comparison analysis. Qualitative and quantitative analysis of the off-odor compounds in muskmelon juice with and without added catechin, genistein, daidzein, phlorotannins or pyrogallol was conducted using headspace solid phase microextraction (HS-SPME) followed by gas chromatography-mass spectrometry (GC-MS), and orthogonal partial least squares discriminant analysis (OPLS-DA) was used to characterize the inhibitory action of each polyphenol on the off-odor compounds. The results revealed that the sensory evaluation was consistent with the instrumental analysis of the types of inhibited off-odor compounds and the percentage inhibition. The five polyphenols at the same concentration of 0.1 mg/mL had different inhibitory effects on the formation of thermally-induced off-odors. Catechin exhibited the strongest inhibitory effect, followed by phlorotannins, daidzein, genistein and pyrogallol, and the effects of catechin, phlorotannins and daidzein were significant (P < 0.05). The combined use of catechin and daidzein could serve as the best way to prevent and minimize the generation of heat-induced off-odors in muskmelon juice. This study provides theoretical guidance to overcome the problem of heat-induced off-odors in muskmelon juice as a representative of thermosensitive fruits and vegetables and to develop deeply processed products.

Key words: muskmelon; thermal processing; off-notes; polyphenols; off-odor inhibition

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