食品科学 ›› 2020, Vol. 41 ›› Issue (14): 88-94.doi: 10.7506/spkx1002-6630-20190725-333

• 生物工程 • 上一篇    下一篇

基于GC-TOF-MS代谢组学研究高度黑糯米酒后发酵阶段代谢差异

姜 丽,苏 伟,母应春,王洪琳,赵 驰   

  1. (贵州大学酿酒与食品工程学院,贵州省发酵工程与生物制药重点实验室,贵州 贵阳 550025)
  • 发布日期:2020-07-29
  • 基金资助:
    国家自然科学基金地区科学基金项目(31860441)

Metabolic Differences in Post-Fermentation Stage of High Black Glutinous Rice Wine Based on GC-TOF-MS Metabolomics

JIANG Li, SU Wei, MU Yingchun, WANG Honglin, ZHAO Chi   

  1. (Guizhou Key Laboratory of Fermentation Engineering and Biopharmacey, School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China)
  • Published:2020-07-29

摘要: 为了解高度黑糯米酒后发酵过程中代谢产物的差异及其变化,采用气相色谱-飞行时间质谱联用仪分别对发酵长达6 个月的酒样进行跟踪监测,并对所得数据进行多元统计分析。结果表明:主成分模型显示发酵1、4、5、6 个月后的代谢产物之间存在明显分离。基于正交偏最小二乘法-判别分析结果发现,共有40 种代谢物被鉴定为差异代谢物(变量重要性投影值>1,P<0.05),其中包括糖类及其衍生物11 种,有机酸13 种,氨基酸7 种,酮类5 种,其他代谢物4 种。对差异代谢物进行通路分析发现,具有显著影响(影响值>1,P<0.05)的代谢通路为甘油脂代谢、半胱氨酸和甲硫氨酸代谢、淀粉和蔗糖代谢、精氨酸和脯氨酸代谢、丙氨酸、天冬氨酸和谷氨酸代谢、三羧酸循环、谷氨酸、亮氨酸和异亮氨酸生物合成、甘油磷脂代谢。

关键词: 高度黑糯米酒;后发酵;代谢组学;差异代谢物

Abstract: This study aimed to analyze the differences and changes of metabolites during the fermentation process of high black glutinous rice. Gas chromatography-time of flight mass spectrometry was used to track and monitor the wine samples after 6 months of fermentation, and the data were analyzed in multivariate statistics. The results showed that the principal component model showed significant differences in metabolites after fermentation for 1, 4, 5, 6 months (P < 0.05). Based on orthogonal partial least squares discriminant analysis, a total of 40 metabolites were identified as differential metabolites (VIP > 1, P < 0.05), including 11 sugars and sugar derivatives, 13 organic acids, 7 amino acids, 5 ketones, and 4 other metabolites. Pathway analysis of differentially metabolites showed that the metabolic pathways that were significantly affected (impact value > 1 and P < 0.05) were glycerolipid metabolism, cysteine and methionine metabolism, starch and sucrose metabolism, arginine and proline metabolism, alanine, aspartate and glutamate metabolism, TCA cycle, valine, leucine and isoleucine biosynthesis, and glycerophospholipid metabolism.

Key words: high black glutinous rice wine; post-fermentation; metabolomic; differential metabolite

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