绿豆肽对脂多糖诱导急性肺损伤小鼠肺组织的保护作用

doi:10.7506/spkx1002-6630-20190822-232

食品科学 ›› 2020, Vol. 41 ›› Issue (17): 176-181.doi: 10.7506/spkx1002-6630-20190822-232

绿豆肽对脂多糖诱导急性肺损伤小鼠肺组织的保护作用

刁静静，刘妍兵，李朝阳，于笛，左锋，张丽萍

（1.黑龙江八一农垦大学国家杂粮工程技术研究中心，黑龙江大庆 163319；2.黑龙江八一农垦大学农业农村部农产加工品质量监督检验测试中心，黑龙江大庆 163319；3.黑龙江八一农垦大学食品学院，黑龙江大庆 163319）

出版日期:2020-09-15 发布日期:2020-09-16
基金资助:
“十三五”国家重点研发计划项目（2017YFD0400200）；黑龙江八一农垦大学校内培育项目（XZR2017-17）

Protective Effect of Mung Bean Protein Hydrolysate on Lipopolysaccharide-Induced Acute Lung Injury in Mice

DIAO Jingjing, LIU Yanbing, LI Zhaoyang, YU Di, ZUO Feng, ZHANG Liping

(1. Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 2. Daqing Center of Inspection and Testing for Agricultural Products, Ministry of Agriculture and Rural Affairs, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 3. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China)

Online:2020-09-15 Published:2020-09-16

摘要/Abstract

摘要： 为评价绿豆肽（mung bean protein hydrolysate，MBPH）在机体中的抗炎作用，采用鼻腔滴注脂多糖法构建急性肺损伤小鼠模型，通过小鼠肺组织病理学变化、肺组织湿干质量比、髓过氧化物酶（myeloperoxidase，MPO）活力、细胞因子表达量、肺泡灌洗液（bronchoalveolar lavage fluid，BALF）的蛋白质量浓度和中性粒细胞数等指标评价MBPH的抗炎作用。结果表明：MBPH能缓解肺组织的损伤，改善模型组肺组织的状态，且MBPH呈剂量依赖性的方式降低肺组织湿/干质量比及其MPO活力，降低BALF的蛋白质量浓度、促炎性细胞因子的含量和中性粒细胞的数目；高剂量MBPH组与5 mg/（kg mb·d）地塞米松组对于急性肺损伤小鼠的抗炎效果相近。结论：MBPH具有抗炎作用，其抗炎作用可能是通过减轻肺水肿和肺微血管渗漏、改善肺组织的病理学变化、减少BALF中中性粒细胞浸润及抑制炎性细胞分泌细胞因子来发挥其对急性肺损伤的保护作用。

关键词: 绿豆肽；脂多糖；急性肺损伤

Abstract: This investigation aims to evaluate the in vitro anti-inflammatory effect of mung bean protein hydrolysate (MBPH). A mouse model of acute lung injury (ALI) was constructed by intranasal instillation of lipopolysaccharide (LPS), and the anti-inflammatory of MBPH was evaluated by assessing pulmonary histopathological changes, the ratio between wet mass and dry mass, myeloperoxidase (MPO) activity, and cytokine concentrations, protein concentration and neutrophil count in bronchoalveolar lavage fluid (BALF). The results showed that MBPH relieved lung injury in a dose-dependent manner, reduced the wet/dry mass ratio and MPO activity of lung tissue, and decreased the concentrations of proteins, pro-inflammatory cytokines and neutrophils in BALF. The anti-inflammatory effect of high-dose MBPH was similar to that of dexamethasone when administered at a dose of 5 mg/(kg mb · d). The above findings indicated that MBPH has an anti-inflammatory effect, which can exert its protective effect on ALI by reducing pulmonary edema and pulmonary microvascular leakage, improving the pathological changes of lung tissue, reducing neutrophil infiltration in BALF, and inhibiting the secretion of cytokines by inflammatory cells.

Key words: mung bean protein hydrolysate; lipopolysaccharide; acute lung injury

中图分类号:

TS214.9

刁静静，刘妍兵，李朝阳，于笛，左锋，张丽萍. 绿豆肽对脂多糖诱导急性肺损伤小鼠肺组织的保护作用[J]. 食品科学, 2020, 41(17): 176-181.

DIAO Jingjing, LIU Yanbing, LI Zhaoyang, YU Di, ZUO Feng, ZHANG Liping. Protective Effect of Mung Bean Protein Hydrolysate on Lipopolysaccharide-Induced Acute Lung Injury in Mice[J]. FOOD SCIENCE, 2020, 41(17): 176-181.

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绿豆肽对脂多糖诱导急性肺损伤小鼠肺组织的保护作用

Protective Effect of Mung Bean Protein Hydrolysate on Lipopolysaccharide-Induced Acute Lung Injury in Mice

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