食品科学 ›› 2020, Vol. 41 ›› Issue (17): 208-215.doi: 10.7506/spkx1002-6630-20190908-103

• 营养卫生 • 上一篇    下一篇

木犀草素及其黄酮苷的抗炎、抗氧化作用

王伟,何平,江小明   

  1. (武汉食品化妆品检验所,湖北 武汉 430012)
  • 出版日期:2020-09-15 发布日期:2020-09-16

Anti-inflammatory and Antioxidant Effects of Luteolin and Its Flavone Glycosides

WANG Wei, HE Ping, JIANG Xiaoming   

  1. (Wuhan Institute for Food and Cosmetic Control, Wuhan 430012, China)
  • Online:2020-09-15 Published:2020-09-16

摘要: 目的:以木犀草素及其3 种黄酮苷(木犀草苷、荭草素、异荭草素)为研究对象,基于HTP-1细胞探究其抗炎与抗氧化活性。方法:采用荧光定量聚合酶链式反应法和Western Blot法分析4 种黄酮类化合物预处理后细胞因子表达情况,核转录因子κB(nuclear factor κB,NF-κB)信号通路调控蛋白κB抑制因子α亚基(α inhibitor of κB,IκBα)、IκB激酶β亚基(IκB kinase β,IKKβ)、P65的表达情况,以及抗氧化相关核因子E2相关性因子2(nuclear factor erythroid 2 related factor 2,Nrf2)及血红素氧合酶1(heme oxygenase 1,HO-1)的表达情况。结果:4 种黄酮类化合物对肿瘤坏死因子(tumor necrosis factor-α,TNF-α)、促炎因子白细胞介素(interleukin,IL)-6均具有极显著抑制作用(P<0.01),荭草素可以进一步抑制促炎因子IL-1β的表达(P<0.01);木犀草素及3 种黄酮苷均可以显著抑制炎性细胞中IKKβ、IκBα的磷酸化,3 种黄酮苷还可减少细胞核中P65的表达量,进而较好地抑制NF-κB信号通路发挥抗炎活性;荭草素可极显著促进炎性细胞中Nrf2的表达(P<0.01),促进Nrf2发生核移位(P<0.01),使HO-1的表达量处于较低水平(P<0.01),保持较低的氧化应激水平。结论:木犀草素及3 种黄酮苷具有较好的抗炎作用,其中荭草素可通过抑制NF-κB信号通路调控蛋白IKKβ、IκBα、P65的表达发挥较强的抗炎活性,显著降低细胞中TNF-α、IL-6、IL-1β的表达,同时通过促进核转录因子Nrf2的表达与核移位,激活Nrf2发挥较好的抗氧化作用,使细胞中HO-1酶表达量处于较低水平。

关键词: 木犀草素;荭草素;抗炎;抗氧化

Abstract: The anti-inflammatory and antioxidant activities of luteolin, cynaroside, orientin and homoorientin were explored on THP-1 cells. The expression of cytokines in the cells pretreated with each of the four flavonoids were analyzed by fluorescence quantitative polymerase chain reaction (PCR) and Western Blot, and the expression of IκB kinase β (IKKβ), α inhibitor of κB (IκBα), NF-κB P65, nuclear factor erythroid 2 related factor 2 (Nrf2) and heme oxygenase 1 (HO-1) enzyme were also analyzed. It was found that the expression of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were extremely significantly inhibited by each of the four flavonoids (P < 0.01). Orientin also inhibited the expression of IL-1β (P < 0.01). These four flavonoids significantly inhibited the phosphorylation of IKKβ and IκBα in inflammatory cells, and the three luteolin glycosides also reduced the content of P65 in the nuclei, thereby exerting their anti-inflammatory effects through the NF-κB signaling pathway. Moreover, orientin extremely significantly promoted the expression and nuclear translocation of Nrf2 in inflammatory cells (P < 0.01), which resulted in a lower expression of HO-1 (P < 0.01) and a lower level of oxidative stress. In conclusion, luteolin and its three derivatives are effective against inflammation. In particular, orientin can down-regulate the expression of the NF-κB signaling pathway-related proteins IKKβ, IκBα and P65, thereby exerting its strong anti-inflammatory activity by significantly reducing the expression of TNF-α, IL-6 and IL-1β. Furthermore, by promoting Nrf2 expression and nuclear translocation, orientin can exert a good antioxidant effect, leading to a low level of HO-1 expression.

Key words: luteolin; orientin; anti-inflammatory; antioxidant

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