关键词: 牛至；多酚；迷迭香酸；诱导

Abstract: Origanum vulgare cells were suspension-cultured to synthesize polyphenols, which were further identified by ultra-high performance liquid chromatography-time of flight-tandem mass spectrometry (UPLC-TOF-MS/MS). Aspergillus niger elicitor (ANE) was used to promote polyphenol synthesis in the cultured cells, and the effects of elicitor concentration, addition time and subsequent culture duration on cell growth and polyphenols synthesis were investigated as well. A total of six polyphenols in the cultured cells were identified, including procatechin-O-hexanoside, salidroside, salicylate-O-glucoside, rosmarinic acid-3-O-glucoside, rosmarinic acid (ROA) and methyl rosmarinate, with the most abundant being ROA as a landmark phenolic compound. Adding 50 μg/mL ANE at the mid-log phase (the 10th day) hardly affected the growth or morphology of cells cultured for another two days, while it promoted polyphenol synthesis, resulting in a 3.25-fold and 3.33-fold increase in the content and production (reaching 361.38 mg/L) of ROA with a simultaneous 2.90-fold increase in the production of total phenolics. Our results indicated that suspension-cultured O. vulgare cells could synthesize a variety of polyphenols. ANE treatment can effectively enhance the polyphenol content in O. vulgare cells, and therefore, is an effective method to improve the polyphenol yield including ROA as a representative component.

Key words: Origanum vulgare; polyphenols; rosmarinic acid; elicitation