食品科学 ›› 2021, Vol. 42 ›› Issue (17): 248-254.doi: 10.7506/spkx1002-6630-20200526-319

• 专题论述 • 上一篇    下一篇

2’-岩藻糖基乳糖的微生物合成研究进展

瓮茹茹,卫鑫慧,李浩正,路福平,李玉   

  1. (天津科技大学生物工程学院,省部共建食品营养与安全国家重点实验室,工业微生物教育部重点实验室 天津 300457)
  • 发布日期:2021-09-29
  • 基金资助:
    国家自然科学基金面上项目(31871741)

Progress in Microbial Synthesis of 2’-Fucosyllactose

WENG Ruru, WEI Xinhui, LI Haozheng, LU Fuping, LI Yu   

  1. (State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China)
  • Published:2021-09-29

摘要: 2’-岩藻糖基乳糖(2’-fucosyllactose,2’-FL)是母乳寡糖中含量最高的寡糖(约占31%),在婴幼儿的生长发育中起重要作用。2019年底,2’-FL已经在欧盟获批作为新型食品投入市场,因此,如何高效、安全地生产2’-FL成为当下的研究热点。与其他方法相比,利用微生物细胞工厂进行2’-FL的合成具有成本低、安全、快捷等特点,是实现大规模生产2’-FL的可行方法。本文主要针对微生物合成2’-FL过程中关键酶的活性、产物的分泌和积累、底物和中间代谢产物的分解代谢以及辅因子再生等方面进行综述,并对未来的发展趋势进行展望。

关键词: 2’-岩藻糖基乳糖;微生物合成;代谢途径改造

Abstract: 2’-Fucosyllactose (2’-FL) is the most abundant human milk oligosaccharides (about 31%), which plays an important role in the growth and development of infants. At the end of 2019, 2’-FL was approved as a new food ingredient for marketing in the European Union. Therefore, how to produce 2’-FL efficiently and safely has become a research hotspot nowadays. Compared with other methods for the synthesis of 2’-FL, using a microbial cell factory has the advantages of low cost, safety and quickness, which is a feasible method for the mass production of 2’-FL. This brief review focuses on the activity of the key enzymes involved in the biosynthesis of 2’-FL, the secretion and accumulation of products, the catabolism of substrates and intermediate metabolites, and the regeneration of cofactors during the process, and it also discussed future trends in this field.

Key words: 2’-fucosyllactose; microbial synthesis; metabolic pathway modification

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