食品科学 ›› 2021, Vol. 42 ›› Issue (20): 230-237.doi: 10.7506/spkx1002-6630-20200610-134

• 安全检测 • 上一篇    下一篇

UPLC-MS/MS检测肉制品中16 种杂环胺前处理方法筛选

丁晓倩,惠腾,白雪,王振宇,张德权   

  1. (中国农业科学院农产品加工研究所,农业农村部农产品加工综合性重点实验室,北京 100193)
  • 出版日期:2021-10-25 发布日期:2021-11-12
  • 基金资助:
    国家自然科学基金青年科学基金项目(31901718);“十三五”国家重点研发计划重点专项(2019YFC1606200)

Screening of Extraction Methods for Use in the Detection of 16 Heterocyclic Amines in Meat Products by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

DING Xiaoqian, HUI Teng, BAI Xue, WANG Zhenyu, ZHANG Dequan   

  1. (Key Laboratory of Agro-products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China)
  • Online:2021-10-25 Published:2021-11-12

摘要: 采用串联固相萃取法(Extrelut-PRS-C18固相萃取柱萃取法、PRS-C18固相萃取柱萃取法)、MCX固相萃取柱萃取法和分散固相萃取法(QuEChERS(quick, easy, cheap, effective, rugged, and safe)法)4 种前处理方法对肉制品中16 种杂环胺(heterocyclic amines,HAs)进行提取,并从方法学和提取成本角度综合比较4 种方法。结果表明:4 种提取方法得到的16 种HAs的回收率和精密度及检出限(limit of detection,LOD)和定量限(limit of quantification,LOQ)存在显著差异,其中QuEChERS法回收率和精密度均优于其他3 种方法。MCX固相萃取柱萃取法和QuEChERS法的LOD和LOQ优于其他2 种方法;MCX固相萃取柱萃取法和QuEChERS法所需经济和时间成本较低。综合分析,QuEChERS法具有快速、简便、便宜、有效、稳固和安全的特点,有利于肉制品中HAs的准确检测,并值得推广应用。

关键词: 杂环胺;肉制品;回收率;固相萃取;QuEChERS法

Abstract: In this work, two tandem solid phase extraction (SPE) methods (Extrelut-PRS-C18 and PRS-C18 SPE columns), MCX SPE column extraction and dispersive SPE (quick, easy, cheap, effective, rugged, and safe (QuEChERS)) were used to extract 16 heterocyclic amines in meat products for analysis by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), and the four methods were comprehensively compared in terms of analytical figures of merit and cost. The results showed that the recoveries, precision, limits of detection (LOD) and limits of quantification (LOQ) for the 16 HAs were significantly different among the methods. The QuEChERS method was superior to the other methods in terms of recovery and precision. The LOD and LOQ of MCX SPE column extraction and QuEChERS were better than those of the other methods. Both MCX and QuEChERS extraction methods showed lower cost and were more time-saving. In summary, the QuEChERS method can allow accurate detection of HAs in meat products and has the potential for wide application.

Key words: heterocyclic amines; meat products; recovery; solid phase extraction; quick, easy, cheap, effective, rugged, and safe

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