食品科学 ›› 2021, Vol. 42 ›› Issue (11): 1-7.doi: 10.7506/spkx1002-6630-20200617-232

• 基础研究 •    下一篇

含酪氨酸二肽在脂质体体系中的抗氧化活性与供电子能力

陈冲,赵谋明,孙为正   

  1. (华南理工大学食品科学与工程学院,广东 广州 510641)
  • 出版日期:2021-06-15 发布日期:2021-06-29
  • 基金资助:
    国家自然科学基金面上项目(31671870);广州市科技计划项目(201803010080;201807010102); 高等学校学科创新引智计划项目(B17018)

Antioxidant Activity and Electron-Donating Capacity of Tyr-Containing Dipeptides in Liposomal Systems

CHEN Chong, ZHAO Mouming, SUN Weizheng   

  1. (School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China)
  • Online:2021-06-15 Published:2021-06-29

摘要: 采用紫外波段扫描对5 种含酪氨酸二肽(酪氨酸-酪氨酸、酪氨酸-苯丙氨酸、苯丙氨酸-酪氨酸、酪氨酸-丙氨酸和酪氨酸-丝氨酸)的紫外吸收特性进行检测,并采用还原力法、循环伏安法及2,2-偶氮二(2-甲基丙基咪)二盐酸盐诱导氧化脂质体模型对其抗氧化活性进行评价及比较,以探究影响5 种含酪氨酸二肽抗氧化活性的因素及其在均相与非均相体系中的活性差异与联系。结果表明:构成含酪氨酸二肽的氨基酸组成及排列顺序对于二肽的紫外吸收特征、供电子能力以及脂质氧化抑制率有显著影响,其中酚羟基的数量对其抗氧化活性起决定性作用。与酪氨酸-苯丙氨酸相比,苯丙氨酸-酪氨酸在3 种抗氧化活性评价方法中均显示出更高的活性。在脂质体体系中,含酪氨酸二肽的脂质氧化抑制率除了与其供电子能力显著相关(P<0.05)外,还与其自身的亲脂或亲水性具有一定的相关性。

关键词: 抗氧化;酪氨酸;二肽;脂质体;循环伏安法

Abstract: The ultraviolet (UV) absorption characteristics of five Tyr-containing dipeptides (Tyr-Tyr, Tyr-Phe, Phe-Tyr, Tyr-Ala, and Tyr-Ser) were investigated, and their antioxidant activities were evaluated and compared using reducing power assay, cyclic voltammetry and 2,2’-azobis(2-amidinopropane)dihydrochloride (AAPH)-induced liposomal oxidation system. The aim was to explore their influential factors and the distinction and correlation between the antioxidant activities in a homogeneous system and those in a heterogeneous system. The results showed that the amino acid composition and sequence of these dipeptides had a significant influence on their UV absorption characteristics, electron-donating capacity and lipid oxidation inhibitory effect, while the amount of phenolic hydroxyl group played a determinant role in the antioxidant activity. Compared with Tyr-Phe, Phe-Tyr exhibited higher activity in the three antioxidant assays. In the liposomal system, the inhibition of lipid oxidation by these dipeptides were significantly correlated (P < 0.05) with their electron-donating capacity as well as their lipophilicity or hydrophilicity.

Key words: antioxidant; tyrosine; dipeptide; liposome; cyclic voltammetry

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