花生主要过敏原Ara h 1线性B细胞表位的预测及鉴定

doi:10.7506/spkx1002-6630-20200701-008

食品科学 ›› 2021, Vol. 42 ›› Issue (17): 106-112.doi: 10.7506/spkx1002-6630-20200701-008

花生主要过敏原Ara h 1线性B细胞表位的预测及鉴定

王俊娟，李欣芮，陈成，孙善峰，刘桂蓉，车会莲

（中国农业大学食品科学与营养工程学院，食品质量与安全北京实验室，北京 100083）

发布日期:2021-09-29
基金资助:
国家自然科学基金面上项目（31972185）

Prediction and Identification of Linear B Cell Epitope on the Major Peanut Allergen Ara h 1

WANG Junjuan, LI Xinrui, CHEN Cheng, SUN Shanfeng, LIU Guirong, CHE Huilian

(Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China)

Published:2021-09-29

摘要/Abstract

摘要： 花生过敏由于其高致敏率和致敏严重性而引起了人们的广泛关注。Ara h 1是花生中的主要过敏原，属于Cupin超家族，通过抗原表位识别结合免疫球蛋白E引发花生过敏。本研究采用生物信息学分析花生主要过敏原Cupin超家族Ara h 1线性B细胞表位氨基酸组成，及其与Ara h 1二级、三级结构之间关系，通过质谱分析Ara h 1氨基酸序列中的抗消化肽段，并分析抗消化肽段与预测线性B细胞表位的关系。结果表明，Ara h 1的线性B细胞表位富含亲水性氨基酸和带电氨基酸；其二级结构没有明显的分布规律，具有一定的回转折叠结构；分析Ara h 1三级结构发现，表位主要位于单体之间的疏水相互作用区域，部分表位埋入三聚体构象内部；Ara h 1抗消化序列与表位之间存在部分重叠。综上，质谱检测体外模拟胃肠道消化肽段并结合表位生物信息学分析可以作为鉴定Cupin超家族线性B细胞表位的新方法。

关键词: 花生过敏；Ara h 1；线性B细胞表位；抗消化肽；生物信息学分析

Abstract: Recently, there has been widespread concern about peanut allergy because of the high sensitization rate to peanut and peanut-induced anaphylaxis. Ara h 1 is a major allergen in peanut that belongs to the Cupin superfamily and can trigger peanut allergies through recognizing and binding to immunoglobulin E (IgE). In this study, bioinformatics was used to analyze the amino acid composition of the linear B cell epitope on peanut allergen Ara h 1 and its correlation with the secondary and tertiary structures of the allergen. The digestion resistant peptides in the Ara h 1 sequence were analyzed by mass spectrometry (MS), and the relationship between the peptides and the predicted linear B cell epitopes was analyzed. The results showed that the linear B cell epitope on Ara h 1 was rich in hydrophilic and charged amino acids. In the secondary structure, the epitope showed no regular distribution pattern but with tuning and folding conformations. In the tertiary structure, it was mainly located in the hydrophobic interaction area between the monomers, partly buried in the trimer conformation. There was a partial overlap between the digestion resistant peptide sequence and the predicted epitope. Detecting peptides from simulated gastrointestinal digestion in vitro by MS combined with bioinformatics analysis can be used as a new method to identify the linear B cell epitope on the Cupin superfamily.

Key words: peanut allergy; Ara h 1; linear B cell epitope; digestion resistant peptide; bioinformatics analysis

中图分类号:

TS201.6

王俊娟，李欣芮，陈成，孙善峰，刘桂蓉，车会莲. 花生主要过敏原Ara h 1线性B细胞表位的预测及鉴定[J]. 食品科学, 2021, 42(17): 106-112.

WANG Junjuan, LI Xinrui, CHEN Cheng, SUN Shanfeng, LIU Guirong, CHE Huilian. Prediction and Identification of Linear B Cell Epitope on the Major Peanut Allergen Ara h 1[J]. FOOD SCIENCE, 2021, 42(17): 106-112.

[1]	肖志文武涌陈红兵. 腰果过敏研究进展[J]. 食品科学, 0, (): 0-0.
[2]	何静胡鑫月. 基于量子区块链的食品冷链追溯系统构建[J]. 食品科学, 0, (): 0-0.
[3]	肖叶叶精勤李晓晨李晓晖施文正卢瑛. 植物乳杆菌（Lactobacillus plantarum）对过敏原原肌球蛋白免疫活性的消减作用[J]. , 0, (): 0-0.
[4]	山珊黄艳梅赵雪龙陈文瑶刘成伟龙中儿刘道峰. 级联信号转导系统结合免疫层析法检测大肠埃希氏菌O157:H7[J]. , 0, (): 0-0.
[5]	杨建飞马倩左勇黄雪芹徐佳王定丽周耀进杨柯怡. 亚硝酸盐快速检测试剂的研究及其在酱腌菜中的应用[J]. , 0, (): 0-0.
[6]	贾飞闫文杰戴瑞彤刘毅李兴民. 基于还原氧化石墨烯/碳纳米管-纳米金复合纳米材料的阻抗型电化学适配体传感器检测铜绿假单胞菌[J]. , 0, (): 0-0.
[7]	刘翼翔马玉李东慧王彦波傅玲琳刘光明. 膳食结构和成分通过调控肠道菌群影响食物过敏的研究进展[J]. , 0, (): 0-0.
[8]	张晋豪王浩东邵良婷徐幸莲王虎虎. 生鲜肉新鲜度快速新型表征技术研究进展[J]. 食品科学, 0, (): 0-0.
[9]	段锦淼高庆超李亚辉张志勇梁颖. 我国与国际组织婴幼儿食品安全标准中风险因子限量差异分析与思考[J]. 食品科学, 0, (): 0-0.
[10]	张慧杨瑞利刘芳周赛楠卢娜唐庆娟. 食品级κ-卡拉胶对肥胖小鼠致结肠炎风险及体脂蓄积的影响[J]. , 0, (): 0-0.
[11]	李琳琼，洪静，张丽君，高瑀珑. 酸胁迫对鼠伤寒沙门氏菌抗酸性、细胞膜及膜蛋白的影响[J]. 食品科学, 2021, 42(15): 27-36.
[12]	曲劲尧，林煜程，毛馨，卢国柱，袁智鹏，张瑶瑶，尤艳莉，李彦伸. 链格孢霉菌侵染番茄产毒机制[J]. 食品科学, 2021, 42(15): 180-186.
[13]	赵雪靳欣迪刘斌赵旭博. 辣椒粉中黄曲霉菌生长及其产毒规律的预测模型构建[J]. , 0, (): 0-0.
[14]	张宜彩林勤保王亮曾莹钟怀宁陈燕芬李忠. 快餐包装纸中矿物油向固体食品模拟物的迁移[J]. 食品科学, 0, (): 0-0.
[15]	惠博文刘锐李健孙君茂. 生命周期视角下食品碳足迹的评估及案例分析[J]. 食品科学, 0, (): 0-0.

花生主要过敏原Ara h 1线性B细胞表位的预测及鉴定

Prediction and Identification of Linear B Cell Epitope on the Major Peanut Allergen Ara h 1

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价