食品科学 ›› 2021, Vol. 42 ›› Issue (20): 69-74.doi: 10.7506/spkx1002-6630-20200701-022

• 生物工程 • 上一篇    下一篇

凝固型绵羊奶酸奶的发酵特性及活菌数变化

杨续金,侯燕军,呼斯乐,韩苏都毕力格,高爱武   

  1. (1.内蒙古农业大学食品科学与工程学院,内蒙古 呼和浩特 010018;2.内蒙古乐科生物技术有限公司,内蒙古 呼和浩特 010020)
  • 出版日期:2021-10-25 发布日期:2021-11-12
  • 基金资助:
    内蒙古自治区“双一流”学科建设基金项目(SPFC202005)

Fermentation Properties of Set Yogurt Made from Sheep Milk and Changes in Viable Bacterial Count during Cold Storage

YANG Xujin, HOU Yanjun, HU Sile, H Sudublg, GAO Aiwu   

  1. (1. College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China;2. Inner Mongolia Lark Biotechnology Co. Ltd., Hohhot 010020, China)
  • Online:2021-10-25 Published:2021-11-12

摘要: 研究凝固型的绵羊奶与牛奶酸奶发酵特性和贮存期特性,利用pH计、酸度滴定、质构仪、电子舌、活菌计数及感官评价对其pH值、滴定酸度、质构特性、味觉特点、活菌数和感官评分进行分析。结果表明:在发酵过程中绵羊奶与牛奶有相同的pH值变化规律,即前2.5 h下降非常缓慢,之后迅速下降,在凝乳前0.5 h下降变缓直到凝乳结束,达到发酵终点的时间分别为5 h和4.5 h;在4 ℃贮存0~24 d期间,酸奶的pH值都在逐渐下降,牛奶酸奶比绵羊奶酸奶下降更大;滴定酸度方面起始时绵羊奶比牛奶高,达到发酵终点时也明显高于牛奶;质构测定结果为YSM(添加益生菌干酪-张发酵的凝固型绵羊奶酸奶)、JDM(嗜热链球菌与保加利亚乳杆菌共同发酵的经典凝固型绵羊奶酸奶)具有更高的硬度和稠度,黏聚性和黏性指数也显著高于JDN(嗜热链球菌与保加利亚乳杆菌共同发酵的经典凝固型牛奶酸奶),奶的种类为影响酸奶硬度、稠度、黏聚性和黏性指数的主要因素,益生菌干酪-张的添加对这些指标的影响很小;电子舌测定结果为丰富性味感值绵羊奶酸奶明显高于牛奶酸奶,酸感值则低于牛奶酸奶,甜、苦、咸、涩、鲜感值差异不明显;感官评价方面绵羊奶酸奶综合评分高于牛奶酸奶,差异性主要体现在滋味、组织状态和喜爱程度上,在色泽和气味方差异不明显;在4 ℃、21 d贮存期间酸奶的活菌数都在逐渐降低,添加益生菌干酪-张的绵羊奶酸奶活菌数最多,第21天时活菌数分别是普通绵羊奶酸奶和牛奶酸奶的10 倍和100 倍以上,干酪-张对绵羊奶酸奶4 ℃贮存期间活菌数的维持有较好作用。同时,实验结果表明:益生菌干酪-张可以作为发酵剂之一与经典的酸奶发酵剂嗜热链球菌和保加利亚乳杆菌搭配用于凝固型绵羊奶酸奶的制作,以增加其功能价值。

关键词: 绵羊奶;凝固型酸奶;干酪乳杆菌;质构;味觉;雷达图

Abstract: In order to explore the fermentation and storage characteristics of set yogurts made from sheep milk and cow milk, their pH, titratable acidity, texture properties, taste characteristics, viable bacterial population and sensory scores were determined by a pH meter, an acidity titrator, a texture analyzer, an electronic tongue, viable counting and sensory evaluation, respectively. During the fermentation process, the two yogurts showed the same pH trends. The pH decreased slowly in the first 2.5 hours, then dropped rapidly, and finally kept declining slowly from 0.5 h before coagulation until the completion of coagulation. The fermentation was completed after 5 and 4.5 h for fermented sheep milk and cow milk, respectively. During 24 days of storage at 4 ℃, the pH of both fermented milks dropped gradually, and fermented cow milk decreased more than did fermented sheep milk. The titratable acidity of fresh sheep milk was higher than that of fresh cow milk, and the titratable acidity of fermented sheep milk was similarly higher than that of fermented cow milk at the end of fermentation. Moreover, fermented sheep milk containing Lactobacillus paracasei subsp. paracasei Zhang (YSM) and that containing L. bulgaricus and Streptococcus thermophilus (JDM) had higher hardness and consistency, and significantly higher cohesiveness and viscosity than did fermented cow milk containing L. bulgaricus and S. thermophilus (JDN). The type of milk was found to be the main factor that affects the hardness, consistency, viscosity and viscosity of yogurt, while the addition of probiotic L. paracasei subsp. paracasei Zhang had little influence on any of these indicators. The results of electronic tongue revealed that the taste sensation value for richness of fermented sheep milk was significantly higher than that of fermented cow milk, while the sourness sensation value was lower that of fermented cow milk. There was no significant difference in the sensation scores for sweet, bitter, salty, astringent or umami taste between the two fermented milks. The overall sensory score of fermented sheep milk was higher than that of fermented cow milk, and there were differences in the sensory scores for taste, texture, and liking degree but not color or smell between them. Total viable bacterial count gradually declined during the 21-day storage period for all fermented milk samples. YSM showed the highest viable bacterial count and its value on day 21 of storage was over 10- and 100-fold higher than that of JDM and JDN, respectively. In conclusion, L. paracasei subsp. paracasei Zhang can be used as a candidate starter culture in combined with the traditional yogurt starter cultures of L. bulgaricus and S. thermophilus for the production of set yogurt made from sheep milk for improved functional value.

Key words: sheep milk; set yogurt; Lactobacillus paracasei subsp. paracasei Zhang; texture; taste;?radar chart

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