食品科学 ›› 2021, Vol. 42 ›› Issue (19): 141-147.doi: 10.7506/spkx1002-6630-20200929-368

• 食品工程 • 上一篇    

低温等离子体对中华管鞭虾(Solenocera crassicorni)菌相变化及品质特性的影响

胡晓梦,陈静,邓尚贵,胡嘉惠   

  1. (1.浙江海洋大学食品与药学学院,浙江 舟山 316022;2.浙江省海产品健康危害因素关键技术研究重点实验室,浙江 舟山 316000)
  • 发布日期:2021-11-12
  • 基金资助:
    国家自然科学基金面上项目(31901764);浙江自然科学基金项目(LY17C200010); 浙江省大学生科技创新活动计划项目(2019R411017)

Effect of Atmospheric Cold Plasma on Microflora and Quality Characteristics of Solenocera crassicorni

HU Xiaomeng, CHEN Jing, DENG Shanggui, HU Jiahui   

  1. (1. College of Food and Medicine, Zhejiang Ocean University, Zhoushan 316022, China; 2. Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, Zhoushan 316000, China)
  • Published:2021-11-12

摘要: 为研究低温等离子体对虾类保鲜贮藏期间的作用机理,本实验以中华管鞭虾为研究对象,利用低温等离子体对其进行处理,考察中华管鞭虾在贮藏过程中菌相及品质变化。以(4±1)℃为贮藏条件,对其微生物多样性进行分析,并对比分析了经低温等离子体处理后中华管鞭虾的挥发性盐基氮(total volatile basic nitrogen,TVB-N)含量、菌落总数、弹性、咀嚼性及肌肉微观结构的变化。结果表明,未处理组中假单胞菌属(Pseudomonas)与腐败希瓦氏菌属(Shewanella)为优势腐败菌,两者相对丰度在贮藏6 d时分别达到49.01%和28.6%,而处理组的优势菌株为气单胞菌属(Aeromonas),贮藏6 d时相对丰度为43.16%;未处理组的TVB-N含量在4 d已经达到(28.03±1.98)mg/100 g,但处理组仅在8 d时接近限值(30 mg/100 g),在物性指标中,处理组与未处理组相比弹性、咀嚼性均下降缓慢;在电子显微镜下观察,在第4天时,未处理组的肌纤维空隙出现絮状结构,而处理组的肌肉微观结构与新鲜状态时无明显差异。综上,低温等离子处理可有效延缓因微生物作用导致的管鞭虾腐败,并显著改变其微观形态,有利于提高贮运品质。本研究结果可为该新型技术应用于水产品保鲜提供理论参考。

关键词: 中华管鞭虾;低温等离子体;微生物多样性;品质特性;微观结构

Abstract: In order to study the mechanism of action of cold plasma in preserving the quality of shrimps during storage, changes in the microflora and quality (microbial diversity, total viable count, total volatile basic nitrogen (TVB-N) content, elasticity, chewiness and microstructure) of Solenocera crassicornis treated by cold plasma was detected during chilled storage at (4 ± 1) ℃. The results showed that Pseudomonas and Shewanella were the dominant spoilage bacteria in untreated samples, whose relative abundance reached 49.01% and 28.6% on day 6, respectively. The dominant strain in treated samples was Aeromonas, whose relative abundance was 43.16% on day 6. The TVB-N content of the control group reached (28.03 ± 1.98) mg/100 g on day 4, while that of the treatment group just approached the maximum limit (30 mg/100 g) set by the national standard of China on the day 8. The elasticity and chewiness of the treated group decreased more slowly compared with the untreated group. As observed under an electron microscope, a flocculent structure appeared in the space among muscle fibers in the untreated group on day 4, while the muscle microstructure of the treated group was not significantly different from that in the fresh state. It is concluded that cold plasma treatment can effectively delay the microbial spoilage of Solenocera crassicornis, and significantly change its microscopic morphology, being beneficial to maintain the product quality during storage and transportation. The results from this study can provide a theoretical reference for the application of this new technology to aquatic product preservation.

Key words: Solenocera crassicorni; cold plasma; microbial diversity; quality characteristics; microstructure

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