食品科学 ›› 2022, Vol. 43 ›› Issue (3): 169-177.doi: 10.7506/spkx1002-6630-20210429-416

• 营养卫生 • 上一篇    下一篇

水溶性豆渣酸性多糖对RAW264.7细胞的免疫调节作用及其机制

胡彦波,王思琪,石曾卉,翟丽媛,赵珺   

  1. (长春大学食品科学与工程学院,吉林 长春 130022)
  • 出版日期:2022-02-15 发布日期:2022-03-08
  • 基金资助:
    吉林省科技厅重点研发项目(20200402061NC);长春大学第二批“攀登计划”项目(ZKP202107)

Immunomodulatory Effect and Mechanism of Soluble Acidic Polysaccharides from Soybean Dregs on RAW264.7 Cells

HU Yanbo, WANG Siqi, SHI Zenghui, ZHAI Liyuan, ZHAO Jun   

  1. (School of Food Science and Engineering, Changchun University, Changchun 130022, China)
  • Online:2022-02-15 Published:2022-03-08

摘要: 研究水溶性豆渣多糖(water-soluble soybean polysaccharides,SSPS)对RAW264.7细胞的免疫调节作用。首先,以NO浓度为指标,明确SSPS、水溶性豆渣酸性多糖A1(soluble soybean acidic polysaccharide A1,SSPS-A1)和水溶性豆渣酸性多糖A1的c组分(fraction c of SSPS-A1,SSPS-A1-c)具有激活RAW264.7巨噬细胞释放一氧化氮的能力。进一步以酸性多糖的纯组分SSPS-A1-c为对象,研究SSPS-A1-c的免疫调节作用及其机制。结果表明,SSPS-A1-c可以激活RAW264.7细胞释放NO和活性氧(reactive oxygen species,ROS),促进其分泌肿瘤坏死因子-α(tumor necrosis factor α,TNF-α)、白细胞介素(interleukin,IL)-1β和IL-6等免疫调节因子,并呈现剂量依赖性。此外,SSPS-A1-c可以促进RAW264.7细胞中丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPKs)相关蛋白c-Jun N-末端激酶(c-Jun N-teminal kinase,JNK)、细胞外信号调节激酶(extracellular regulated protein kinases,ERK)和蛋白激酶-38(phosphor-38,p38)的磷酸化和核因子-κB(nuclear factor kappa B,NF-κB)信号通路中核因子κB抑制蛋白-α(nuclear factor-kappa-B inhibitor alpha,IκB-α)的降解,说明SSPS-A1-c通过MAPKs和NF-κB信号通路发挥免疫调节作用。上述研究结果说明水溶性豆渣酸性多糖SSPS-A1-c对RAW264.7细胞具有较好的免疫调节作用。这将为豆渣多糖的综合开发利用提供新的思路,同时为食品功能性因子提供候选分子。

关键词: 水溶性豆渣酸性多糖;巨噬细胞;免疫调节;丝裂原活化蛋白激酶;核因子-κB

Abstract: The immunomodulatory effect of soluble soybean polysaccharides (SSPS) from soybean dregs on RAW264.7 cells was studied in this work. It was clear that SSPS, soluble soybean acidic polysaccharide A1 (SSPS-A1) and a purified fraction c from SSPS-A1 (SSPS-A1-c) could activate RAW264.7 macrophages to release nitric oxide (NO). Furthermore, the immunomodulatory effect and mechanism of action of SSPS-A1-c were clarified. The results showed that SSPS-A1-c could promote RAW264.7 cells to release NO and reactive oxygen species (ROS), and stimulate the secretion of tumor necrosis factor α (TNF-α), interleukin (IL)-1β and IL-6 in a dose-dependent manner. In addition, SSPS-A1-c could promote the phosphorylation of the mitogen-activated protein kinase (MAPK)-related protein kinases c-Jun N-terminal kinase (JNK), extracellular regulated protein kinases (ERK) and phosphor-38 (p38), as well as the degradation of nuclear factor-kappa-B inhibitor alpha (IκB-α) in the nuclear factor kappa B (NF-κB) signaling pathway, suggesting that SSPS-A1-c played an immunomodulatory role through the MAPKs and NF-κB signaling pathway. These findings indicate that SSPS-A1-c has a good immunomodulatory effect on RAW264.7 cells. This study will provide a new idea for the comprehensive development and utilization of water-soluble polysaccharides from soybean dregs, and provide candidate molecules for functional food factors.

Key words: soluble acidic polysaccharides; macrophage; immunoregulation; mitogen-activated protein kinase; nuclear factor-κB

中图分类号: