食品科学 ›› 2022, Vol. 43 ›› Issue (9): 95-102.doi: 10.7506/spkx1002-6630-20210429-418

• 营养卫生 • 上一篇    下一篇

两种泽兰酸性多糖的结构和生物活性

张武霞,胡懿化,何嘉琦,王星滟,赵晋忠,李鹏   

  1. (山西农业大学基础部,中兽医药现代化山西省重点实验室,山西 太谷 030801)
  • 出版日期:2022-05-15 发布日期:2022-05-27
  • 基金资助:
    国家自然科学基金青年科学基金项目(31800678);山西农业大学科技创新基金项目(2017YJ40); 山西省高等学校科技创新项目(No.2021L146);山西省重点研发计划重点项目(201903D211006-1); 山西省研究生教育创新项目(2021Y349)

Structures and Biological Activities of Two Acidic Polysaccharides from Lycopi Herba

ZHANG Wuxia, HU Yihua, HE Jiaqi, WANG Xingyan, ZHAO Jinzhong, LI Peng   

  1. (Shanxi Key Lab for Modernization of TCVM, Department of Basic Sciences, Shanxi Agricultural University, Taigu 030801, China)
  • Online:2022-05-15 Published:2022-05-27

摘要: 从泽兰中提取多糖组分,并评价其体外抗氧化活性和细胞免疫调节活性。通过DEAE-52纤维素柱层析和SephadexG-100柱层析从泽兰粗多糖中分离得到2 个纯多糖,分别命名为LHPS1和LHPS5。采用物理化学方法和免疫学方法对LHPS1和LHPS5的结构和生物活性进行了研究。结果表明,LHPS1(1.13×105 Da)和LHPS5(7.40×104 Da)是由多种单糖和半乳糖醛酸组成的酸性多糖。总还原能力和1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除率测定结果表明,LHPS1和LHPS5具有显著的体外抗氧化活性。免疫实验分析结果表明,LHPS1和LHPS5能激活RAW264.7细胞促进肿瘤坏死因子(tumor necrosis factor,TNF)-α的产生,并促进脾淋巴细胞增殖。荧光免疫激活转运实验结果显示LHPS1和LHPS5能够通过激活RAW264.7细胞中的核因子κB(nuclear factor kappa-B,NF-κB)调节免疫应答。此外,抑制剂中和实验结果显示两种多糖可能通过结合细胞表面甘露糖受体激活巨噬细胞。LHPS1和LHPS5具有一定的体外抗氧化活性和细胞免疫调节活性,可用作抗氧化补充剂或免疫调节剂。

关键词: 泽兰;多糖;抗氧化活性;免疫调节活性

Abstract: The antioxidant activity and cellular immunomodulatory activity of polysaccharide fractions obtained from Lycopi Herba were evaluated in vitro. Two homogenous polysaccharides, designated LHPS1 and LHPS5, were isolated from the crude polysaccharide extract of Lycopi Herba by DEAE-52 and SephadexG-100 column chromatography. The structures and biological activities of LHPS1 and LHPS5 were studied by physicochemical and immunological methods. The results showed that LHPS1 (1.13 × 105 Da) and LHPS5 (7.40 × 104 Da) were acidic polysaccharides, composed of many monosaccharides and galacturonic acid. In terms of total reducing power and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity, both polysaccharides had significant antioxidant activity in vitro. Immunoassays indicated that they could activate RAW264.7 cells to produce tumor necrosis factor-α (TNF-α), and promote the proliferation of splenic lymphocytes. Fluorescence immuno-activated transport assay showed that they could regulate immune responses by activating the transcription factor nuclear factor-kappa B (NF-κB) in RAW264.7 cells. In addition, inhibitor neutralization test results showed that the two polysaccharides could activate macrophages by binding to cell surface mannose receptors. Overall, we concluded that LHPS1 and LHPS5 had antioxidant activity and cellular immunomodulatory activity in vitro, and could be used as antioxidant supplements or immunomodulatory agents.

Key words: Lycopi Herba; polysaccharides; antioxidant activity; immunoregulatory activity

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