紫苏籽多糖分离纯化及抗肿瘤活性

doi:10.7506/spkx1002-6630-20210708-087

食品科学 ›› 2022, Vol. 43 ›› Issue (15): 158-165.doi: 10.7506/spkx1002-6630-20210708-087

• 营养卫生 • 上一篇

紫苏籽多糖分离纯化及抗肿瘤活性

刘子坤，尹贺，杨安皓，安绮沄，李冲伟，金涛

（1.黑龙江大学农业微生物技术教育部工程研究中心，黑龙江哈尔滨 150500；2.黑龙江大学生命科学学院，黑龙江哈尔滨 150080；3.哈尔滨美苏生物科技开发有限公司，黑龙江哈尔滨 150080）

发布日期:2022-08-30
基金资助:
2019年度哈尔滨市雏鹰计划暨科技创业奖补专项（2019CYJBCG0326）

Separation, Purification and Anti-tumor Activity of Polysaccharides from Perilla frutescens Seeds

LIU Zikun, YIN He, YANG Anhao, AN Qiyun, LI Chongwei, JIN Tao

(1. Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500, China; 2. College of Life Sciences, Heilongjiang University, Harbin 150080, China; 3. Harbin MeiSu Biotechnology Development Co., Ltd., Harbin 150080, China)

Published:2022-08-30

摘要/Abstract

摘要： 为了探究紫苏籽多糖（Perilla frutescens seed polysaccharide，PFSP）的抗肿瘤活性，利用传统方法从紫苏籽中提取粗多糖，利用DEAE-52纤维素和葡聚糖G-100柱层析分离纯化获得紫苏籽多糖，并测定该多糖对H22荷瘤小鼠免疫系统的影响。结果表明，紫苏籽粗多糖得率为8.38%，分离纯化后得到3 个组分，分别命名为PFSP-1、PFSP-2和PFSP-3。PFSP-1由甘露糖、半乳糖、木糖及阿拉伯糖（物质的量之比为0.01∶0.06∶0.11∶0.81）组成，PFSP-2由甘露糖、木糖及阿拉伯糖（物质的量之比为0.28∶0.28∶0.41）组成，PFSP-3由鼠李糖、葡萄糖醛酸、葡萄糖、半乳糖、木糖及阿拉伯糖（物质的量之比为0.013∶0.024∶0.040∶0.080∶0.120∶0.700）组成；3 个组分均具有多糖特征吸收峰，为吡喃环型多聚糖。抗肿瘤实验表明，PFSP-2抑瘤率显著高于PFSP-1和PFSP-3（P＜0.05）；对PFSP-2进一步研究发现，与模型组相比，PFSP-2可显著降低乳酸脱氢酶、醛缩酶和白细胞介素（interleukin，IL）-10质量浓度（P＜0.05），提高IL-2、肿瘤坏死因子α质量浓度（P＜0.05），下调抗凋亡蛋白Bcl-2和上调促凋亡蛋白Bax表达（P＜0.05），说明PFSP-2通过提高小鼠自身免疫能力抑制体内肿瘤细胞的生长。

关键词: 紫苏籽；多糖；分离纯化；免疫；抗肿瘤活性

Abstract: In order to explore the anti-tumor activity of Perilla frutescens seed polysaccharide (PFSP), the crude polysaccharide was obtained from Perilla frutescens seeds by traditional solvent extraction and purified by DEAE-52 cellulose and dextran G-100 column chromatography, and the effect of the purified polysaccharide on the immune system of H22 tumor bearing mice was determined. The results showed that the yield of the crude polysaccharide was 8.38%. After separation and purification, three polysaccharide fractions, named PFSP-1, PFSP-2 and PFSP-3, were obtained. PFSP-1 was composed of mannose, galactose, xylose and arabinose (at a molar ratio of 0.01:0.06:0.11:0.81). PFSP-2 was composed of mannose, xylose and arabinose (at a molar ratio of 0.28:0.28:0.41). PFSP-3 was composed of rhamnose, glucuronic acid, glucose, galactose, xylose and arabinose (at a molar ratio of 0.013:0.024:0.040:0.080:0.120:0.700). The three polysaccharides all displayed absorption peaks characteristic of polysaccharides and were identified as pyran-type polysaccharides. The anti-tumor effect of PFSP-2 was significantly higher than that of PFSP-1 and PFSP-3 (P < 0.05). Furthermore, PFSP-2 could significantly reduce the concentrations of lactate dehydrogenase and aldolase and interleukin (IL)-10 (P < 0.05), increase the concentrations of IL-2 and tumor necrosis factor α, down-regulate the expression of antiapoptotic protein Bcl-2, and up-regulate the expression of apoptotic protein Bax compared with the model group (P < 0.05). These results indicate that PFSP-2 can inhibit the growth of tumor cells in vivo by improving the immune system of mice.

Key words: Perilla frutescens seed; polysaccharide; separation and purification; immune; anti-tumor activity

中图分类号:

TS241

刘子坤，尹贺，杨安皓，安绮沄，李冲伟，金涛. 紫苏籽多糖分离纯化及抗肿瘤活性[J]. 食品科学, 2022, 43(15): 158-165.

LIU Zikun, YIN He, YANG Anhao, AN Qiyun, LI Chongwei, JIN Tao. Separation, Purification and Anti-tumor Activity of Polysaccharides from Perilla frutescens Seeds[J]. FOOD SCIENCE, 2022, 43(15): 158-165.

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紫苏籽多糖分离纯化及抗肿瘤活性

Separation, Purification and Anti-tumor Activity of Polysaccharides from Perilla frutescens Seeds

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