食品科学 ›› 2022, Vol. 43 ›› Issue (17): 188-198.doi: 10.7506/spkx1002-6630-20210823-298

• 营养卫生 • 上一篇    下一篇

亚油酸/α-亚麻酸复合物对小鼠急性肝损伤的预防作用

沙爽，冯启鑫，张欣蕊，王玥，尹贺，李冲伟   

  1. （1.黑龙江大学 农业微生物技术教育部工程研究中心，黑龙江 哈尔滨 150500；2.黑龙江大学生命科学学院，黑龙江 哈尔滨 150080；3.哈尔滨美苏生物科技开发有限公司，黑龙江 哈尔滨 150080）
  • 出版日期:2022-09-15 发布日期:2022-09-28
  • 基金资助:
    2019年度哈尔滨市雏鹰计划暨科技创业奖补专项（2019CYJBCG0326）

Preventive Effect of Linoleic Acid and α-Linolenic Acid Mixtures on Acute Liver Injury in Mice

SHA Shuang, FENG Qixin, ZHANG Xinrui, WANG Yue, YIN He, LI Chongwei   

  1. (1. Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500, China; 2. College of Life Sciences, Heilongjiang University, Harbin 150080, China; 3. Harbin Meisu Biotechnology Development Co., Ltd., Harbin 150080, China)
  • Online:2022-09-15 Published:2022-09-28

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摘要： 为探究亚油酸/α-亚麻酸复合物对急性肝损伤小鼠的肝脏细胞和肠道微生物多样性的影响，将80 只昆明SPF小鼠分为8 组，分别为正常组、CCl4模型组、阳性对照组以及亚油酸/α-亚麻酸复合物1∶4组、1∶2组、1∶1组、2∶1组和4∶1组，测定8 组实验小鼠血清免疫功能指标和肠道细菌菌群结构。结果表明，CCl4诱导小鼠急性肝损伤模型建立成功；与模型组相比，5 种复合物均可降低肝损伤小鼠血清谷草转氨酶（aspartate aminotransferase，AST）和谷丙转氨酶（alanine aminotransferase，ALT）活力（P＜0.05），降低肝脏指数和脾脏指数（P＞0.05），降低肝组织丙二醛（malondialdehyde，MDA）含量（P＜0.05），降低肝组织坏死因子（tumor necrosis factor-α，TNF-α）和白细胞介素（interleukin，IL）-6表达水平（P＜0.05），这些指标变化与肝组织病理切片结果完全对应。高通量测序结果表明，与普通组相比，CCl4模型组小鼠肠道细菌多样性降低（P＜0.05），亚油酸/α-亚麻酸复合物2∶1组与CCl4模型组相比小鼠肠道细菌多样性和丰度呈显著上升趋势（P＜0.05），肠道细菌菌群结构发生改变。同时发现，亚油酸/α-亚麻酸为2∶1时对肝脏的保护效果优于其他比例，与水飞蓟宾阳性对照组效果基本一致。本实验结果不仅证明了亚油酸/α-亚麻酸复合物对急性肝损伤小鼠肝脏的保护作用，而且为亚油酸/α-亚麻酸的科学摄入提供了理论依据，可为护肝药物的开发和应用提供新的思路。

关键词: α-亚麻酸；亚油酸；急性肝损伤；肝脏保护；肠道菌群

Abstract: This study aimed to investigate the effect of linoleic acid and α-linolenic acid mixtures on acute liver injury and the intestinal microflora diversity in mice. In total 80 specific pathogen free (SPF) Kunming mice were divided into eight groups: normal, CCl4-induced model, positive control, and linoleic acid/α-linolenic acid mixtures at ratios of 4:1, 2:1, 1:1, 1:2 and 1:4. Serum immune functions and intestinal microflora structure in each group of mice were determined. The results showed that linoleic acid and α-linolenic acid mixtures could significantly reduce the activity of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) (P < 0.05), decreased liver and spleen indices (P > 0.05), and decreased the content of malondialdehyde (MDA) as well as the expression levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in liver cells (P < 0.05) compared with CCl4-induced model . These changes were consistent with the results of liver histopathological observation. High throughput sequencing results showed that the diversity of intestinal microflora in the model group dropped significantly compared with normal group (P < 0.05), while the diversity and abundance of intestinal microflora significantly increased after intervention with the 2:1 mixture of linoleic acid/α-linolenic acid compared with the CCl4 model group (P < 0.05), and its structure also changed. Moreover, the most prominent hepatoprotective effect was observed with the 2:1 mixture of linoleic acid and α-linolenic acid, which was basically consistent with that of silybin as a positive control. These results not only confirm the hepatoprotective effect of linoleic acid/α-linolenic acid mixtures, but also provide a theoretical basis for the scientific intake of linoleic acid/α-linolenic acid mixtures and serve as a guide for future development and application of hepatoprotective agents.

Key words: α-linolenic acid; linoleic acid; acute liver injury; liver protection; intestinal flora

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