食品科学 ›› 2022, Vol. 43 ›› Issue (21): 233-240.doi: 10.7506/spkx1002-6630-20211211-133

• 营养卫生 • 上一篇    

荔枝果壳原花青素对中波紫外线诱导HaCaT细胞氧化损伤的保护作用

董丽红,罗牡康,张名位,张瑞芬,邓梅,陈燕霞,贾栩超   

  1. (广东省农业科学院蚕业与农产品加工研究所,农业农村部功能食品重点实验室,广东省农产品加工重点实验室,广东 广州 510610)
  • 发布日期:2022-12-12
  • 基金资助:
    广州市科技计划项目(201903010051;202103000055);广东特支计划项目(2019BT02N112); 广东省农业科学院农业优势产业学科团队建设项目(202108TD)

Protective Effect of Procyanidins from Litchi Pericarp on Ultraviolet B-Induced Oxidative Damage in HaCaT Cells

DONG Lihong, LUO Mukang, ZHANG Mingwei, ZHANG Ruifen, DENG Mei, CHEN Yanxia, JIA Xuchao   

  1. (Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Agricultural Products Processing, Sericultural & Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China)
  • Published:2022-12-12

摘要: 为评价荔枝果壳原花青素对中波紫外线(ultraviolet B,UVB)(波长280~320 nm)诱导人永生化角质形成细胞(HaCaT)氧化损伤的保护作用。构建UVB辐射HaCaT细胞氧化损伤模型,研究荔枝果壳低聚原花青素(litchi pericarp oligomolymeric procyanidins,LPOPC)及从中分离鉴定的6 种单体化合物对HaCaT细胞氧化损伤的保护作用。实验分为对照组、UVB照射组、实验组(阳性对照对氨基苯甲酸(para-aminobenzoic acid,PABA)、LPOPC及6 种单体化合物),以CCK-8法测定各组细胞活力,采用试剂盒检测各组细胞内活性氧自由基(reactive oxygen species,ROS)相对含量,超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力,还原型谷胱甘肽(glutathione,GSH)及丙二醛(malondialdehyde,MDA)含量。结果表明:LPOPC及6 种单体化合物的干预处理均可明显提高UVB诱导氧化损伤的HaCaT细胞活力,减少细胞内ROS和MDA的生成,增加SOD、CAT、GSH-Px的活力和GSH水平。6 种单体化合物中,原花青素A2的保护作用最明显,与阳性药物PABA的效果相当。结论:LPOPC及6 种单体化合物均可通过增强细胞内抗氧化能力、抑制脂质过氧化来明显改善受损细胞氧化应激损伤,对UVB诱导氧化损伤的HaCaT细胞具有良好的保护作用,提示原花青素A2是荔枝果壳原花青素中对氧化应激损伤防护作用最强的活性组分。

关键词: 荔枝果壳原花青素;人永生化角质形成细胞;中波紫外线辐射;氧化应激损伤;保护作用

Abstract: The protective effect of oligomolymeric procyanidins (LPOPC) and six monomeric procyanidins from litchi pericarp on ultraviolet B (UVB, 280–320 nm)-induced oxidative damage in HaCaT cells was evaluated in this study. UVB-damaged HaCaT cells were treated with para-aminobenzoic acid (PABA) as a positive control, LPOPC and the monomeric procyanidins, separately. Native and irradiated cells without drug treatment were used as a control group and a model group, respectively. Cell viability was detected by the CCK-8 method. The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) were detected using commercial kits, and the levels of reactive oxygen species (ROS), glutathione (GSH) and malondialdehyde (MDA) were also measured. These results showed that LPOPC and the six monomeric compounds significantly increased the viability of HaCaT cells damaged by UVB, reduced the generation of intracellular ROS and MDA, increased the activities of SOD, CAT and GSH-Px and GSH levels. The protective effect of procyandin A2 against oxidative stress in HaCaT cells was the most significant among the six monomeric compounds, which was equivalent to that of PABA. Therefore, LPOPC and the monomeric compounds have a good protective effect on UVB-induced oxidative damage in HaCaT cells by enhancing intracellular antioxidant capacity and inhibiting lipid peroxidation. It is suggested that procyanidin A2 from litchi pericarp is an active component with the strongest protective effect.

Key words: procyandins from litchi pericarp; HaCaT cells; ultraviolet B radiation; oxidative stress-induced damage; protective effect

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