关键词: 过敏反应；大鼠嗜碱性粒细胞；转录组测序；差异表达基因；信号通路

Abstract: IgE-mediated rat basophilic leukemia (RBL)-2H3 cells were used to construct a cell model of anaphylaxis. RNA sequencing was used to analyze differentially expressed genes between unactivated and activated RBL-2H3 cells, and the related signaling pathways were enriched. The activation of IgE-mediated RBL-2H3 cells significantly increased the secretion of allergenic mediators and cytokines. Totally 232 differentially expressed genes were obtained by RNA sequencing, of which 127 (54.74%) could be annotated for gene function. Meanwhile, the activation of RBL-2H3 could affect the tumor necrosis factor, mitogen-activated protein kinase, janus kinase-signal transducer and activator of transcription, and Toll-like receptor signaling pathways. The transcription factors involved in these signaling pathways included MAP3K8, Nfkbia, Junb, Jun and Fos. In conclusion, the findings of this study may provide a theoretical foundation for the prevention and treatment of allergic diseases.

Key words: anaphylactic reaction; rat basophilic leukemia-2H3; RNA sequencing; differential expressed genes; signaling pathways