葡萄柚籽提取物及其纳米乳对大肠杆菌和金黄色葡萄球菌生物菌膜的抑制作用

doi:10.7506/spkx1002-6630-20220111-098

食品科学 ›› 2023, Vol. 44 ›› Issue (3): 22-30.doi: 10.7506/spkx1002-6630-20220111-098

葡萄柚籽提取物及其纳米乳对大肠杆菌和金黄色葡萄球菌生物菌膜的抑制作用

王思亓，梁晓云，张晨，张文栋，程宇，宓晓雨，赵王晨，王龙凤，江芸

（南京师范大学食品与制药工程学院，江苏?南京 210023）

出版日期:2023-02-15 发布日期:2023-02-28
基金资助:
国家自然科学基金面上项目（32072287）；江苏省自然科学基金青年项目（BK20190700）

Inhibitory Effects of Grapefruit Seed Extract and Its Nanoemulsion on Biofilm Formation of Escherichia coli and Staphylococcus aureus

WANG Siqi, LIANG Xiaoyun, ZHANG Chen, ZHANG Wendong, CHENG Yu, MI Xiaoyu, ZHAO Wangchen, WANG Longfeng, JIANG Yun

(School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, China)

Online:2023-02-15 Published:2023-02-28

摘要/Abstract

摘要： 目的：研究葡萄柚籽提取物（grapefruit seed extract，GSE）及其纳米乳（grapefruit seed extract nanoemulsion，GNE）对大肠杆菌和金黄色葡萄球菌生物菌膜的抑制作用。方法：分析菌膜黏附菌数变化、胞外聚合物（extracellular polymeric substances，EPS）含量并进行微观结构观察，比较GSE和GNE对两菌菌膜（单种菌膜和混合菌膜）形成的抑制作用和已形成菌膜的清除效果。结果：与对照组相比，1/2最小抑菌浓度（minimum inhibitory concentration，MIC）GSE和GNE可以抑制两菌单种菌膜形成时黏附菌和EPS产生，且对金黄色葡萄球菌的抑制作用更强。扫描电子显微镜（scanning electron microcopy，SEM）和共聚焦激光扫描显微镜（confocal laser scanning microscope，CLSM）所观察到的微观结构变化也显示出GSE和GNE对两菌菌膜形成具有抑制作用。GSE和GNE对已形成菌膜也有良好清除效果，且对金黄色葡萄球菌菌膜的清除作用更强，但混合菌膜的抗性强于单种菌膜。比较GSE和GNE发现，GNE的菌膜形成抑制及清除效果均强于GSE，1/2 MIC GNE作用120 h时两单菌菌膜形成量对数值均比GSE组少0.6（lg（CFU/cm2））以上，质量分数1.8% GNE对菌膜的清除率比GSE高7.7%～27.2%。结论：GSE和GNE对大肠杆菌和金黄色葡萄球菌生物菌膜具有一定的抑制作用，研究可为GSE和GNE在生物菌膜控制方面的推广应用提供一定的科学依据。

关键词: 葡萄柚籽提取物；纳米乳；大肠杆菌；金黄色葡萄球菌；生物菌膜

Abstract: Purpose: To investigate the inhibitory effects of grapefruit seed extract (GSE) and its nanoemulsion (GNE) on biofilm formation of Escherichia coli and Staphylococcus aureus. Methods: The inhibitory effects of GSE and GNE on the formation of single and mixed-species biofilms were explored by analyzing the changes in the number of adherent cells and the content of extracellular polymeric substances (EPS) as well as microstructure observation, and the effectiveness of GSE and GNE in eliminating biofilms formed by E. coli and S. aureus was compared. Results: The number of adherent cells and EPS production during single-species biofilm formation were inhibited by GSE and GNE at 1/2 minimum inhibitory concentration (MIC), and S. aureus was more strongly inhibited. The changes of biofilm microstructure observed under a scanning electron microscope (SEM) and a confocal laser scanning microscope (CLSM) confirmed the inhibitory effect of GSE and GNE on biofilm formation by the two bacteria. GSE and GNE could also eliminate E. coli and S. aureus biofilms, and were more effective for S. aureus biofilm, but the resistance of the mixed-species biofilms was stronger than that of the single-species biofilms. GNE was more effective than GSE in inhibiting biofilm formation and eliminating biofilms. The amount of single-species biofilms formed by the two bacteria decreased by 0.6 (lg (CFU/cm2)) when treated with GNE at 1/2 MIC for 120 h compared with GSE, and the elimination rate of biofilms by 1.8% GNE increased by 7.7%–27.2% compared with GSE. Conclusion: GSE and GNE have inhibitory effect on on biofilm formation of Escherichia coli and Staphylococcus aureus. This study can provide a scientific basis for the application of GSE and GNE in biofilm control.

Key words: grapefruit seed extract; nanoemulsion; Escherichia coli; Staphylococcus aureus; biofilm

中图分类号:

TS201.3

王思亓，梁晓云，张晨，张文栋，程宇，宓晓雨，赵王晨，王龙凤，江芸. 葡萄柚籽提取物及其纳米乳对大肠杆菌和金黄色葡萄球菌生物菌膜的抑制作用[J]. 食品科学, 2023, 44(3): 22-30.

WANG Siqi, LIANG Xiaoyun, ZHANG Chen, ZHANG Wendong, CHENG Yu, MI Xiaoyu, ZHAO Wangchen, WANG Longfeng, JIANG Yun. Inhibitory Effects of Grapefruit Seed Extract and Its Nanoemulsion on Biofilm Formation of Escherichia coli and Staphylococcus aureus[J]. FOOD SCIENCE, 2023, 44(3): 22-30.

[1]	李玉蝶李玟君汪海燕宋青云庞子皓肖一郎汪超李玮. 戊糖乳杆菌发酵对花生不同蛋白组分结构的影响[J]. 食品科学, 0, (): 0-0.
[2]	傅晓莹乔玮博史硕博. 微生物利用一碳底物生产单细胞蛋白研究进展[J]. , 0, (): 0-0.
[3]	胡娜利佳炜闫珍珍陈雄李欣. 蛋白质定量质谱技术及其在食品加工用酵母中的应用[J]. , 0, (): 0-0.
[4]	傅晓莹，乔玮博，史硕博. 微生物利用一碳底物生产单细胞蛋白研究进展[J]. 食品科学, 2023, 44(3): 1-11.
[5]	胡娜，利佳炜，闫珍珍，陈雄，李欣. 蛋白质定量质谱技术及其在食品加工用酵母中的应用[J]. 食品科学, 2023, 44(3): 12-21.
[6]	马岩，王中江，杨靖瑜，李哲，彭霞，单秀峰，李柏良，马微微. 动物双歧杆菌乳亚种XLTG11对克林霉素诱导的抗生素相关性腹泻的改善作用[J]. 食品科学, 2023, 44(3): 170-178.
[7]	涂明霞，刘蕾，高亮，张颖，桂萌，饶瑜. 气单胞菌群体感应及其抑制剂研究进展[J]. 食品科学, 2023, 44(3): 350-358.
[8]	马岩王中江杨靖瑜李哲彭霞单秀峰李柏良马微微. 动物双歧杆菌乳亚种XLTG11对克林霉素诱导的抗生素相关性腹泻的改善作用[J]. 食品科学, 0, (): 0-0.
[9]	王思亓梁晓云张晨张文栋程宇宓晓雨赵王晨王龙凤江芸. 葡萄柚籽提取物及其纳米乳对大肠杆菌和金黄色葡萄球菌生物菌膜的抑制作用[J]. 食品科学, 0, (): 0-0.
[10]	王润东周奎邓义佳张宇昊李学鹏励建荣. 鱼油激活结肠上皮PPAR信号缓解副溶血弧菌诱导的小鼠肠炎损伤[J]. 食品科学, 0, (): 0-0.
[11]	贾丽娜马倩王新月葛艳艳王楠何红鹏罗学刚朱丽颖贾卫国郝力壮齐威. 基于体外发酵模型分析植物多糖对T2DM患者肠道菌群及代谢的影响[J]. 食品科学, 0, (): 0-0.
[12]	吴惠香，杨华，赵登奇，黄建颖，杨利军. 三甲胺柱[5]芳烃的合成及其抑菌性能[J]. 食品科学, 2023, 44(1): 46-52.
[13]	赵青刘欣牛洪梅朱华剑潘馨叶刘阳泰杨捷琳董庆利. 食源性致病菌VBNC诱导、复苏及检测的研究进展[J]. 食品科学, 0, (): 0-0.
[14]	鲁重刘静聪吴诗媛冯立芳陈剑朱军莉. 生鲜食品致腐假单胞菌生物被膜形成及其调控机制研究进展[J]. 食品科学, 0, (): 0-0.
[15]	周巧张梦梅刘书亮李建龙胡凯弟李琴. 丝状真菌降解拟除虫菊酯类农药和3-苯氧基苯甲酸研究进展[J]. 食品科学, 0, (): 0-0.

葡萄柚籽提取物及其纳米乳对大肠杆菌和金黄色葡萄球菌生物菌膜的抑制作用

Inhibitory Effects of Grapefruit Seed Extract and Its Nanoemulsion on Biofilm Formation of Escherichia coli and Staphylococcus aureus

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价