基于体外发酵模型分析植物多糖对T2DM患者肠道菌群及代谢的影响

doi:10.7506/spkx1002-6630-20220111-102

食品科学 ›› 2023, Vol. 44 ›› Issue (2): 248-261.doi: 10.7506/spkx1002-6630-20220111-102

• 生物工程 • 上一篇

基于体外发酵模型分析植物多糖对T2DM患者肠道菌群及代谢的影响

贾丽娜，马倩，王新月，葛艳艳，王楠，何红鹏，罗学刚，朱立颖，贾卫国，郝力壮，齐威

（1.天津科技大学生物工程学院，天津 300457；2.浙江省农业科学院，浙江杭州 310021；3.四川省第五人民医院，四川成都 610014；4.青海省高原放牧家畜动物营养与饲料科学重点实验室，青海省牦牛工程技术研究中心，青海大学畜牧兽医科学院，青海西宁 810016）

发布日期:2023-01-31
基金资助:
青海省高原放牧家畜动物营养与饲料科学重点实验室项目（2022-ZJ-Y17）； “十三五”国家重点研发计划重点专项（2018YFA0901700）；青海省“昆仑英才·高端创新创业人才”拔尖人才项目（2020）

Effects of Plant Polysaccharides on Intestinal Flora and Metabolism in Patients Investigated Using in Vitro Fermentation Model

JIA Lina, MA Qian, WANG Xinyue, GE Yanyan, WANG Nan, HE Hongpeng, LUO Xuegang, ZHU Liying, JIA Weiguo, HAO Lizhuang, QI Wei

(1. College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China; 2. Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 3. The Fifth People’s Hospital of Sichuan Province, Chengdu 610014, China;4. Key Laboratory of Plateau Grazing Animal Nutrition and Feed Science of Qinghai Province, Qinghai Yak Engineering Technology Research Center, Qinghai Academy of Animal Science and Veterinary Medicine, Xining 810016, China)

Published:2023-01-31

摘要/Abstract

摘要： 通过体外发酵模型，利用高通量测序及代谢组学技术，研究5 种植物多糖（黄芪多糖、苍术多糖、玄参多糖、红枣多糖和乌梅多糖）对2型糖尿病（type 2 diabetes mellitus，T2DM）患者肠道微生物及其代谢产物的影响。结果表明，植物多糖能够显著改善糖尿病人肠道菌群结构，并且5 种多糖均能够富集Faecalibacterium、Roseburia，抑制Escherichia。植物多糖处理能够调节气体介质含量、短链脂肪酸含量，改变T2DM患者肠道菌群的氨基酸、短链脂肪酸代谢途径，其中黄芪多糖、苍术多糖和玄参多糖对短链脂肪酸总量调节作用显著，对乙酸、丙酸、丁酸、戊酸、异丁酸和异戊酸含量具有一定的调节作用；黄芪多糖、苍术多糖、玄参多糖和红枣多糖均能够提高T2DM患者的总气体介质含量以及CO2和CH4含量，乌梅多糖降低了总气体介质含量和H2S含量。研究结果为植物多糖的筛选与利用及其靶向调节肠道微生物提供了理论基础。

关键词: 植物多糖；2型糖尿病；肠道菌群；代谢物质

Abstract: This study aims to investigate the effects of five plant polysaccharides from Radix Astragalus, Rhizoma Atractylodis, Radix Scrophulariae, jujubes, Fructus mume on intestinal microorganisms and their metabolites in patients with type 2 diabetes mellitus (T2DM) by using high-throughput sequencing, metabolomic techniques and an in vitro fermentation model. The results showed that plant polysaccharides could significantly improve the structure of the intestinal flora in diabetic patients, and all five polysaccharides could enrich Faecalibacterium and Roseburia and inhibit Escherichia. Plant polysaccharides could regulate the contents of gas medium and short-chain fatty acids, and change the metabolic pathways of amino acids and short-chain fatty acids in the intestinal flora of patients with T2DM. Radix Astragalus, Atractylodis Rhizoma and Radix Scrophularia polysaccharides had significant regulatory effects on the total amount of short-chain fatty acids, and could also regulate the contents of acetic acid, propionic acid, butyric acid, valeric acid, isobutyric acid and isovaleric acid. In addition to these polysaccharides, jujube polysaccharide also increased the content of total gaseous media as well as CO2 and CH4 in patients with T2DM, while Fructus mume polysaccharide could reduce the contents of gas medium and H2S. These results provide a theoretical basis for the screening and utilization of plant polysaccharides and understanding their targeted regulation of intestinal microorganisms.

Key words: plant polysaccharides; type 2 diabetes mellitus; intestinal flora; metabolites

中图分类号:

TS201.3

贾丽娜，马倩，王新月，葛艳艳，王楠，何红鹏，罗学刚，朱立颖，贾卫国，郝力壮，齐威. 基于体外发酵模型分析植物多糖对T2DM患者肠道菌群及代谢的影响[J]. 食品科学, 2023, 44(2): 248-261.

JIA Lina, MA Qian, WANG Xinyue, GE Yanyan, WANG Nan, HE Hongpeng, LUO Xuegang, ZHU Liying, JIA Weiguo, HAO Lizhuang, QI Wei. Effects of Plant Polysaccharides on Intestinal Flora and Metabolism in Patients Investigated Using in Vitro Fermentation Model[J]. FOOD SCIENCE, 2023, 44(2): 248-261.

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基于体外发酵模型分析植物多糖对T2DM患者肠道菌群及代谢的影响

Effects of Plant Polysaccharides on Intestinal Flora and Metabolism in Patients Investigated Using in Vitro Fermentation Model

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