食品科学 ›› 2022, Vol. 43 ›› Issue (24): 74-82.doi: 10.7506/spkx1002-6630-20220301-013

• 食品化学 • 上一篇    

球磨联合碱辅助酶法改善淡竹叶水溶性膳食纤维的物化和功能特性

樊华,刘夫国,王玉堂,李银霞,刘学波   

  1. (西北农林科技大学食品科学与工程学院,陕西 杨凌 712100)
  • 发布日期:2022-12-28
  • 基金资助:
    陕西省科技创新团队项目(2019TD-006)

Ball Milling Combined with Alkaline-Assisted Enzymatic Extraction Improved the Physicochemical and Functional Properties of Soluble Dietary Fiber from Herba Lophatheri

FAN Hua, LIU Fuguo, WANG Yutang, LI Yinxia, LIU Xuebo   

  1. (College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China)
  • Published:2022-12-28

摘要: 以淡竹叶为原料,采用酶法、球磨辅助酶法、碱辅助酶法和球磨联合碱辅助酶法4 种方法提取水溶性膳食纤维(soluble dietary fiber,SDF)。测定SDF得率以及通过高效液相色谱仪、纳米激光粒度仪、流变仪、扫描电子显微镜、傅里叶变换红外光谱仪、X射线衍射仪、差示扫描量热仪对4 种SDF的性质进行表征,并对其持水力、持油力、抗氧化性进行比较分析。结果表明:所有SDF均有明显的多糖特征和纤维素结晶类型,但其单糖组成和微观结构不同。球磨联合碱辅助酶法得到的SDF表现出最高的提取率((30.03±1.03)%)和持水力((4.86±0.1)g/g)、高黏度、最低的Zeta电位值((-22.83±1.95)mV);还具有疏松多孔的结构,较好的2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)阳离子自由基清除活性((49.40±0.93)%),表明具有良好的束缚水、抗氧化、形成凝胶的能力。

关键词: 淡竹叶;水溶性膳食纤维;酶;球磨;碱处理

Abstract: Soluble dietary fiber (SDF) was extracted from Herba Lophatheri by enzymatic extraction, ball milling-assisted enzymatic extraction, alkaline-assisted enzymatic extraction, and ball milling combined with alkaline-assisted enzymatic extraction. The yield of SDF was determined, the four SDFs were characterized by high performance liquid chromatography (HPLC), nano-laser particle size analyzer, rheometer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD) and differential scanning calorimetry (DSC), and their water-holding capacity, oil-holding capacity, and antioxidant activity were analyzed and compared. The results suggested that all SDFs had the typical structure of polysaccharides and the same crystal types as cellulose, but their monosaccharide composition and microstructure were different. SDF extracted by ball milling combined with alkaline-assisted enzymatic extraction had the highest yield ((30.03 ± 1.03)%) and water-holding capacity ((4.86 ± 0.1) g/g), high viscosity, and the lowest zeta potential ((?22.83 ± 1.95) mV). Besides, it had a loose porous structure, and potent 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation scavenging activity ((49.40 ± 0.93)%), indicating strong water-binding capacity, antioxidant activity, and gel-forming capacity.

Key words: Herba Lophatheri; soluble dietary fiber; enzyme; ball milling; alkaline treatment

中图分类号: