食品科学 ›› 2023, Vol. 44 ›› Issue (5): 355-364.doi: 10.7506/spkx1002-6630-20220310-125

• 专题论述 • 上一篇    

无细胞生物传感器及其在食品与生物检测应用中的研究进展

王佳佳,王鑫,庞晓旭,黄昆仑,许文涛,罗云波,程楠   

  1. (1.中国农业大学食品科学与营养工程学院,食品质量与安全北京实验室,北京 100083;2.农业农村部农业转基因生物安全评价(食用)重点实验室,北京 100083;3.中国农业大学食品科学与营养工程学院,北京食品营养与人类健康高精尖创新中心,北京 100083)
  • 发布日期:2023-03-23
  • 基金资助:
    中国农业大学2115人才工程项目

Research Progress on Cell-Free Biosensor and Its Application in Food and Biological Detection

WANG Jiajia, WANG Xin, PANG Xiaoxu, HUANG Kunlun, XU Wentao, LUO Yunbo, CHENG Nan   

  1. (1. Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; 2. Key Laboratory of Safety Assessment of Genetically Modified Organism (Food Safety), Ministry of Agriculture and Rural Affairs, Beijing 100083, China; 3. Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China)
  • Published:2023-03-23

摘要: 无细胞生物传感器是一类基于无细胞系统设计的微量物质检测工具,解决了早期活细胞生物传感器无法检测有毒物质、响应时间长、受活细胞控制、操作不便等问题。近年来,无细胞生物传感器以其检测范围广、响应速度快、灵敏度高和便携性好而受到广泛关注。本文按照构建无细胞传感器平台进行分类,并对不同类型无细胞传感器的构建和特点进行综述,进一步介绍其在金属离子、农药与兽药残留等领域检测的研究进展,并对其应用前景进行展望。

关键词: 无细胞生物传感器;检测应用;灵敏度;便携性;液相/纸基生物传感器

Abstract: The cell-free biosensor is a tool designed to detect trace substances based on a cell-free system, which solves the problems of the traditional cell-based biosensor including having no ability to detect toxic substances, long response time, being controlled by living cells, and inconvenient operation. In recent years, cell-free biosensors have attracted extensive attention for their wide detection range, fast response speed, high sensitivity and portability. In this paper, the construction and characteristics of different types of cell-free sensors are reviewed. In addition, their applications in the detection of metal ions, pesticide residues and veterinary drug residues are introduced, and future prospects also are discussed.

Key words: cell-free biosensor; application of testing technologies; sensitivity; portability; liquid phase/paper based biosensor

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