渗透汽化结合SLE-GC-MS分析乙醇对吐鲁浸膏特征香气物质提取的影响

doi:10.7506/spkx1002-6630-20220705-049

食品科学 ›› 2023, Vol. 44 ›› Issue (10): 240-248.doi: 10.7506/spkx1002-6630-20220705-049

渗透汽化结合SLE-GC-MS分析乙醇对吐鲁浸膏特征香气物质提取的影响

姚忠，刘琪，张华，李炜，冒顾政，朱怀远，廖惠云，周浩力

（1.南京工业大学食品与轻工学院，江苏南京 211816；2.江苏中烟工业有限责任公司，江苏南京 210019；3.南京工业大学材料化学工程国家重点实验室，江苏南京 211816）

出版日期:2023-05-25 发布日期:2023-06-02
基金资助:
国家自然科学基金面上项目（21776137）；江苏中烟对外合作科技创新项目（202111）

Effect of Ethanol on the Extraction Efficiency of the Characteristic Aroma Compounds of Tolu Balsam Analyzed by Pervaporation Combined with Solid-Phase Supported Liquid-Liquid Extraction Coupled with Gas Chromatography-Mass Spectrometry

YAO Zhong, LIU Qi, ZHANG Hua, LI Wei, MAO Guzheng, ZHU Huaiyuan, LIAO Huiyun, ZHOU Haoli

(1. College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China; 2. China Tobacco Jiangsu Industrial Co. Ltd., Nanjing 210019, China; 3. State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing 211816, China)

Online:2023-05-25 Published:2023-06-02

摘要/Abstract

摘要： 为探明吐鲁浸膏香气特征及物质组分基础，以5 种不同乙醇体积分数处理的吐鲁浸膏为研究对象，使用渗透汽化膜进行分离处理，对样品溶液进行感官评价和固相支持液液萃取-气相色谱-质谱联用法分析，并利用化学计量学探究样品目标组分差异性和分析感官属性与物质组分之间相关性。结果表明：透过液和截留液以膏香和甜香为主，树脂香、果香、清香和辛香为辅，奶香、花香、豆香和木香香韵相对较弱；气相色谱-质谱共鉴定出92 种物质组分，其中酯类、醇类物质含量较多，其含量占比大于55%，酸类、醛类、酚类、其他类和烯烃类紧随其后，酮类含量相对低；主成分分析和聚类热图分析显示低、中乙醇体积分数和高乙醇体积分数吐鲁浸膏样品分别聚为一类，且前者物质组分含量整体高于后者，正交偏最小二乘判别分析筛选出43 个差异化合物；经偏最小二乘回归确定吐鲁浸膏特征呈香物质与膏香、树脂香、清香和辛香具有显著正相关，而与甜香、果香、奶香、花香、豆香和木香相关性不明显。研究结果为吐鲁浸膏感官品质研究和精细化加工提供技术依据。

关键词: 吐鲁浸膏；渗透汽化；气相色谱-质谱联用；特征香气；香味物质；化学计量学

Abstract: In order to analyze the aroma characteristics and composition of tolu balsam, tolu balsam extracts prepared with five different concentrations of ethanol were separated by pervaporation. The retentate and permeate were evaluated by sensory evaluation and their volatile and semi volatile components were analyzed by solid-phase supported liquid-liquid extraction coupled with gas chromatography-mass spectrometry (SLE-GC-MS). Chemometrics was adopted to explore the difference in target components and the correlation between sensory properties and components. The results showed that the major aroma characteristics of both retentate and permeate were ointment-like and sweet, followed by resin-like, fruity, fresh, and spicy aromas as well as weak creamy, floral, bean-like, and woody aroma notes. A total of 92 components were identified by GC-MS, with esters and alcohols being the predominant ones, accounting for more than 55% of the total, followed by acids, aldehydes, phenols, others and olefins, together with relatively low levels of ketones. Principal component analysis (PCA) and cluster heatmap analysis indicated that tolu balsam extracts obtained with low and medium ethanol concentrations were distinguished from that obtained with high ethanol concentration, and the contents of the identified compounds were overall higher in the former than in the latter. By orthogonal partial least squares discriminant analysis (OPLS-DA), 43 differential compounds were selected. Partial least square regression (PLSR) showed that the characteristic aroma substances of tolu balsam extracts had significant positive correlation with ointment-like, resin-like, fresh and spicy aromas, but had no significant correlation with sweet, fruity, creamy, floral, bean-like or woody aroma. The results of this study may provide a theoretical basis for research on the sensory quality and fine processing of tolu balsam.

Key words: tolu balsam extract; pervaporation; gas chromatography-mass spectrometry; characteristic aroma; volatile components; chemometrics

中图分类号:

TS202.3

姚忠，刘琪，张华，李炜，冒顾政，朱怀远，廖惠云，周浩力. 渗透汽化结合SLE-GC-MS分析乙醇对吐鲁浸膏特征香气物质提取的影响[J]. 食品科学, 2023, 44(10): 240-248.

YAO Zhong, LIU Qi, ZHANG Hua, LI Wei, MAO Guzheng, ZHU Huaiyuan, LIAO Huiyun, ZHOU Haoli. Effect of Ethanol on the Extraction Efficiency of the Characteristic Aroma Compounds of Tolu Balsam Analyzed by Pervaporation Combined with Solid-Phase Supported Liquid-Liquid Extraction Coupled with Gas Chromatography-Mass Spectrometry[J]. FOOD SCIENCE, 2023, 44(10): 240-248.

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渗透汽化结合SLE-GC-MS分析乙醇对吐鲁浸膏特征香气物质提取的影响

Effect of Ethanol on the Extraction Efficiency of the Characteristic Aroma Compounds of Tolu Balsam Analyzed by Pervaporation Combined with Solid-Phase Supported Liquid-Liquid Extraction Coupled with Gas Chromatography-Mass Spectrometry

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