食品科学 ›› 2023, Vol. 44 ›› Issue (13): 88-96.doi: 10.7506/spkx1002-6630-20220712-120

• 营养卫生 • 上一篇    下一篇

石榴汁对葡聚糖硫酸钠诱导小鼠溃疡性结肠炎的作用

吴淑娟,王新军,张武胜,孟卫红,夏禹承,张润光,张有林   

  1. (1.陕西师范大学食品工程与营养科学学院,陕西 西安 710119;2.陕西潼关县红宝石软籽石榴农民专业合作社,陕西 渭南 714300)
  • 出版日期:2023-07-15 发布日期:2023-08-11
  • 基金资助:
    陕西省重点研发计划项目(2022NY-018);中央财政林业科技推广示范项目(SLTG[2021]04号); 陕西省林业科学院科技创新计划专项(SXLK2021-0232)

Effect of Pomegranate Juice on Dextran Sulfate Sodium Salt-Induced Ulcerative Colitis in Mice

WU Shujuan, WANG Xinjun, ZHANG Wusheng, MENG Weihong, XIA Yucheng, ZHANG Runguang, ZHANG Youlin   

  1. (1. College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710119, China; 2. Shaanxi Tongguan County Ruby Soft Seed Pomegranate Farmers’ Professional Cooperatives, Weinan 714300, China)
  • Online:2023-07-15 Published:2023-08-11

摘要: 溃疡性结肠炎是一种发病机制尚不明确且难以根治的慢性病,在我国发病率逐年升高,其药物治疗依赖性强,副作用明显,因此寻求天然营养疗法成为人们的关注热点。本研究采用葡聚糖硫酸钠(dextran sulfate sodium salt,DSS)建立小鼠溃疡性结肠炎模型,用美沙拉嗪(130 mg/(kg mb·d))和石榴汁(30%、100%(体积分数),10 mL/d)进行干预,检测小鼠体质量、疾病活动指数(disease activity index,DAI)、结肠长度,采用试剂盒检测小鼠肝肾功能指标及氧化应激水平,采用酶联免疫吸附剂测定法(enzyme-linked immunosorbent assay,ELISA)检测炎症因子水平,并对结肠组织切片观察,采用气相色谱-质谱联用(gas chromatography-mass spectrometry,GC-MS)分析小鼠粪便短链脂肪酸(short-chain fatty acids,SCFAs)含量,综合评价石榴汁对小鼠溃疡性结肠炎的作用及其机制。结果发现:与DSS组相比,石榴汁干预组小鼠体质量和结肠长度增加、DAI降低、肝肾功能改善,促炎因子表达量极显著降低(P<0.01),抗炎因子表达量极显著提升(P<0.01),氧化应激水平及炎性标志物髓过氧化物酶(myeloperoxidase,MPO)活力极显著降低(P<0.01),粪便SCFAs含量上调,结肠上皮结构改善。石榴汁能改善小鼠肠黏膜损伤和肝肾功能障碍,对DSS诱导的小鼠溃疡性结肠炎具有良好的治疗作用。

关键词: 石榴汁;葡聚糖硫酸钠;小鼠结肠炎;作用机制

Abstract: Ulcerative colitis is a chronic inflammatory bowel disease with unclear pathogenesis and is difficult to cure. The incidence rate is increasing year by year, and there is high drug dependence for the treatment of ulcerative colitis, which leads to obvious the treatment resistance to s side effects. Therefore, seeking natural health products for nutritional therapy has become an attention focus of attention. In this articlestudy, dextran sulfate sodium salt (DSS) was used to establish a mouse model of ulcerative colitis. The mice were intervened with 5-aminosalicylic acid (5-ASA) (130 mg/(kg mb∙d)) or pomegranate juice (30% and 100%, 10 mL/d). Body mass, disease activity index (DAI) and colon length were measured, liver and kidney function and oxidative stress were measured by biochemical kits, the levels of inflammatory factors were measured by enzyme-linked immunosorbent assay (ELISA), colon tissue sections were observed, and the contents of fecal short-chain fatty acids (SCFAs) were determined by gas chromatography-mass spectrometry (GC-MS). Finally, a comprehensive evaluation of the effect and mechanism of pomegranate juice against ulcerative colitis in mice was exploredperformed. Compared with the DSS group, body mass and colon length in the pomegranate juice intervention group was increased, DAI was decreased, liver and kidney functions were improved, and could the expression of proinflammatory factors was significantly decreased (P < 0.01). In addition, anti-inflammatory factor expression was significantly enhanced (P < 0.01), reduce the level of oxidative stress and the activity of inflammatory marker myeloperoxidase (MPO) were significantly reduced (P < 0.01), the level of up-regulate fecal SCFAs was up-regulated, andimprove the colonic epithelial structure was improved. Pomegranate juice can attenuate intestinal mucosal damage and liver and kidney dysfunction, and has a good therapeutic effect on DSS-induced ulcerative colitis in mice.

Key words: pomegranate juice; dextran sulfate sodium; colitis in mice; mechanism

中图分类号: