食品科学 ›› 2023, Vol. 44 ›› Issue (18): 381-388.doi: 10.7506/spkx1002-6630-20220909-089

• 安全检测 • 上一篇    

基于金@铂纳米酶的食品中过氧化氢残留快速检测试纸的研制

李博然,成璐瑶,曹智鸿,程楠,罗云波   

  1. (1.中国农业大学食品科学与营养工程学院,食品质量与安全北京实验室,北京 100083;2.农业农村部农业转基因生物安全评价(食用)重点实验室,北京 100083)
  • 发布日期:2023-09-29
  • 基金资助:
    中国农业大学2115人才工程项目;山东省重点研发计划项目(2019JZZY011014)

Preparation of Test Strip Based on Au@Pt Nanozymes for Rapid Detection of Hydrogen Peroxide Residues in Foods

LI Boran, CHENG Luyao, CAO Zhihong, CHENG Nan, LUO Yunbo   

  1. (1. Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; 2. Key Laboratory of Safety Assessment of Genetically Modified Organism (Food Safety), Ministry of Agriculture and Rural Affairsy, Beijing 100083, China)
  • Published:2023-09-29

摘要: 为实现对食品中残留过氧化氢便捷、灵敏的检测,研制一种基于金@铂纳米酶的过氧化氢快速检测试纸。该试纸以Whatman No.1滤纸为载体,以金@铂纳米酶为催化剂,以3,3’,5,5’-四甲基联苯胺为显色剂,以pH 4.6的醋酸-醋酸钠溶液为缓冲体系制备而成。通过优化试纸制作工艺,确定试纸浸泡时间为10 s,最快显色稳定时间为3 min。该试纸检出限为0.34 mg/kg,具有较好的线性关系(R2=0.993 1)。该试纸制作方便、准确度高、稳定性好、操作简单,可以应用于食品各流通环节的即时现场检测。

关键词: 过氧化氢;快速检测;试纸法;纳米酶;泡椒制品

Abstract: A gold@platinum (Au@Pt) nanozyme-based test strip for the convenient and sensitive detection of hydrogen peroxide residues in foods was developed using Whatman No.1 filter paper as the carrier, Au@Pt nanozyme as the catalyst, 3,3’,5,5’-tetramethylbenzidine as the color developer, and acetic acid-sodium acetate solution at pH 4.6 as the buffer system. The optimized test strip soaking time was 10 s. The test paper could detect hydrogen peroxide in samples within 3 min with a minimum detection limit of 0.34 mg/kg and a good linearity (R2 = 0.993 1). The test paper is easy to make, highly accurate, stable and simple to operate, and can be used in the real-time on-site detection of food products in various distribution channels.

Key words: hydrogen peroxide; rapid detection; test strip; nanozyme; pickled pepper products

中图分类号: