食品科学 ›› 2023, Vol. 44 ›› Issue (18): 324-330.doi: 10.7506/spkx1002-6630-20220915-142

• 安全检测 • 上一篇    

马拉硫磷的纳米金适配体比色传感检测方法

田恒旗,王耀,杨海涛,李兆周,吴佳蓓,王琳,刘浩宇,常云鹤,陈秀金   

  1. (1.河南科技大学食品与生物工程学院,河南 洛阳 471023;2.河南宜测科技有限公司,河南 郑州 451162;3.贵阳学院食品与制药工程学院,贵州 贵阳 550005)
  • 发布日期:2023-09-29
  • 基金资助:
    国家自然科学基金青年科学基金项目(31702218);河南省自然科学基金项目(182300410038;212300410137); 河南省青年人才托举工程项目(2020HYTP029);贵州省自然科学基金项目(ZK[2022]008)

Gold Nanoparticle-based Colorimetric Aptasensor for Detection of Malathion

TIAN Hengqi, WANG Yao, YANG Haitao, LI Zhaozhou, WU Jiabei, WANG Lin, LIU Haoyu, CHANG Yunhe, CHEN Xiujin   

  1. (1. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China; 2. Henan YICE Technology Co. Ltd., Zhengzhou 451162, China;3. College of Food and Pharmaceutical Engineering, Guiyang University, Guiyang 550005, China)
  • Published:2023-09-29

摘要: 基于纳米金(gold nanoparticles,AuNPs)在高盐溶液中会发生团聚而导致溶液颜色发生变化的原理,建立一种新型的马拉硫磷比色传感检测方法。通过优化检测反应体系溶液浓度,得到NaCl最适浓度为500 mmol/L,适配体最适浓度为1 μmol/L。本方法在马拉硫磷质量浓度为50~1 500 ng/mL范围内呈现良好的线性关系,检出限为45.54 ng/mL,检测生菜和苹果样品中马拉硫磷的加标回收率分别为98.24%~100.91%和99.48%~101.29%。结果表明建立的AuNPs-适配体比色传感法可用于马拉硫磷的灵敏、特异、准确、快速检测。本研究为快速检测食品中的马拉硫磷残留提供了一种新方法,并可为其他农药残留的适配体比色传感检测提供参考。

关键词: 纳米金;马拉硫磷;适配体;比色

Abstract: In this study, a novel colorimetric sensing method for the detection of malathion was established based on the principle that gold nanoparticles (AuNPs) can aggregate in high-salt solutions, leading to color changes in the solutions. The optimal concentration of NaCl was 500 mmol/L and the optimal concentration of aptamer was 1 μmol/L in the reaction system. This method showed a good linear relationship in the range of malathion concentration of 50–1 500 ng/mL, with a detection limit of 45.54 ng/mL. The recoveries of malathion in spiked lettuce and apple samples were 98.24%–100.91% and 99.48%–101.29%, respectively. The AuNPs-based colorimetric aptasensor could be used for the sensitive, specific, accurate and rapid detection of malathion. Furthermore, this study may provide a reference for developing colorimetric aptasensors for the detection of other pesticide residues.

Key words: gold nanoparticles; malathion; aptamer; colorimetric

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