食品科学 ›› 2024, Vol. 45 ›› Issue (5): 94-101.doi: 10.7506/spkx1002-6630-20230831-238

• 成分分析 • 上一篇    下一篇

基于电导率的多指标联合技术在蛋液组分检测中的应用

窦文浩,王海岭,黄子龙,杨福明,迟媛,迟玉杰   

  1. (1.东北农业大学食品学院,黑龙江 哈尔滨 150030;2.东北农业大学工程学院,黑龙江 哈尔滨 150030)
  • 出版日期:2024-03-15 发布日期:2024-04-03
  • 基金资助:
    “十四五”国家重点研发计划重点专项(2022YFD2101005);国家蛋鸡产业技术体系项目(CARS-40-K25)

Application of Conductivity Combined with Various Other Instrumental Analyses in the Detection of the Freshness and Yolk-to-White Ratio of Liquid Egg

DOU Wenhao, WANG Hailing, HUANG Zilong, YANG Fuming, CHI Yuan, CHI Yujie   

  1. (1. College of Food Science, Northeast Agricultural University, Harbin 150030, China; 2. College of Engineering, Northeast Agricultural University, Harbin 150030, China)
  • Online:2024-03-15 Published:2024-04-03

摘要: 建立一种以电导率为主,盐度、总溶解性固体(total dissolved solids,TDS)、pH值、色泽为辅的多指标联合技术,实现蛋液新鲜度和调配比的快速、高效检测。结果表明:4 ℃贮藏0~45 d期间,蛋黄液和蛋清液电导率、盐度、TDS呈逐渐上升趋势,二者色泽指标及蛋黄液pH值均显著降低,而蛋清液pH值逐渐升高。检测蛋液新鲜度,发现电导率法检测蛋黄液和蛋清液的准确率分别为91%和89%,电导率协同检测准确率为94%,据此,首次建立了AA、A、B、C级的液蛋新鲜度标准。选定AA级液蛋,以电导率为主要指标,盐度、TDS和pH值为协同指标,检测调配比,通过实验测得电导率、盐度、TDS、pH值与调配蛋液中的蛋黄质量分数的线性拟合回归方程均具有极显著相关性,可根据回归方程检测未知调配比例蛋液,ExpAssoc模型显示模型平均相对误差为8.87%,具有高度可靠性。以此,本实验为调配蛋液检测(新鲜度、调配比)提供了理论依据,为液蛋工业生产加工提供了实际应用的参考。

关键词: 调配蛋液;新鲜度;电导率;快速检测

Abstract: This study established a rapid and efficient technique to detect the freshness and yolk-to-white ratio of liquid egg based on conductivity combined with salinity, total dissolved solids (TDS), pH, and color parameters. The results showed that during the storage period of 0-45 days at 4 ℃, the conductivity, salinity, and TDS of egg yolk and egg white gradually increased. The color difference index and egg yolk pH of both decreased significantly, while the pH of egg white gradually increased. It was found that the accuracy of conductivity in detecting the freshness of egg yolk and egg white was 91% and 89%, respectively, while that of conductivity combined with other indices was 94%. Based on this, the criteria to classify liquid egg into four grades of AA, A, B and C were established for the first time. AA liquid eggs were selected to detect yolk-to-white ratio using conductivity as the major index combined with salinity, TDS and pH. The experimental results proved that the conductivity, salinity, TDS and pH were all highly significantly and linearly correlated with the proportion of egg yolk in liquid egg; therefore, the proportion of egg yolk in unknown liquid egg samples could be detected based on the regression equations. The ExpAssoc model showed an average relative error of 8.87%, indicating high reliability. The results of this study provide a theoretical basis for the detection of the freshness and yolk-to-white ratio of blended liquid egg, and provide a reference for industrial producers of liquid egg.

Key words: blended liquid egg; freshness; conductivity; quick detection

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