食品科学 ›› 2009, Vol. 30 ›› Issue (2 ): 208-210.doi: 10.7506/spkx1002-6630-200902046

• 分析检测 • 上一篇    下一篇

禽蛋中胆固醇的酶催化分光光度测定法研究

葛庆联1,葛庆丰2,刘 迪1,吕晓娟1,朱云芬1,刘学贤1,陈宽维1   

  1. 1.中国农业科学院家禽研究所 2.扬州大学旅游烹饪学院
  • 收稿日期:2008-01-28 修回日期:2008-03-22 出版日期:2009-01-15 发布日期:2010-12-29
  • 通讯作者: 葛庆联 E-mail:zsj10800@sina.com
  • 基金资助:

    农业部公益性行业(农业)科研专项经费子项目(nyhyzx07-039)

Catalytic Spectrophotometry with Enzyme for Determination of Cholesterol in Eggs

GE Qing-lian1,GE Qing-feng2,LIU Di1,LU Xiao-juan1,ZHU Yun-fen1,LIU Xue-xian1,CHEN Kuan-wei1   

  1. (1. Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225003, China;2. College of Tourism Cuisine,
    Yangzhou University, Yangzhou 225002, China)
  • Received:2008-01-28 Revised:2008-03-22 Online:2009-01-15 Published:2010-12-29
  • Contact: GE Qing-lian1 E-mail:zsj10800@sina.com

摘要:

本实验建立一种禽蛋中胆固醇含量快速测定方法——酶催化分光光度法。禽蛋匀浆液由直接皂化法处理,经酶工作液催化后,用分光光度法于波长500nm 处测定其含量。方法回收率均在90% 以上,变异系数均低于4%,线性范围20~600mg/ml,具有良好的准确度与精密度,适合禽蛋中胆固醇含量的快速测定。

关键词: 酶催化分光光度法, 胆固醇, 禽蛋

Abstract:

A simple catalytic spectrophotometic with enzyme method for rapid determination of cholesterol in eggs was developed. Sample was prepared by direct saponification, and then detemined with spectrophotometry at 500 nm after enzyme catalyzed reaction. The recovery of this method is above 90% with RSD below 4% and the linear range is 20 to 600 mg/ml. It was proved that the method is precise and accurate, and so it is suitable for rapid determination of cholesterol in eggs.

Key words: catalytic spectrophotometry with enzyme, cholesterol, egg

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