食品科学 ›› 2025, Vol. 46 ›› Issue (13): 351-305.doi: 10.7506/spkx1002-6630-20250108-054

• 安全检测 • 上一篇    

HPLC-FLD法测定婴幼儿配方奶粉中6 种母乳低聚糖

王弈忻，陈楠楠，曹梦思，杨丽涛，高秀芝，刘明，郭新光   

  1. （1.北京农学院食品科学与工程学院，农业农村部农产品加工与品质控制重点实验室，北京 102206；2.中国食品发酵工业研究院有限公司，北京 100015）
  • 发布日期:2025-06-13
  • 基金资助:
    “十四五”国家重点研发计划重点专项（2022YFF0710403）

Determination of Six Human Milk Oligosaccharides in Infant Formula by High Performance Liquid Chromatography-Fluorescence Detector

WANG Yixin, CHEN Nannan, CAO Mengsi, YANG Litao, GAO Xiuzhi, LIU Ming, GUO Xinguang   

  1. (1. Key Laboratory of Agricultural Product Processing and Quality Control, Ministry of Agriculture and Rural Affairs, College of Food Science and Engineering, Beijing University of Agriculture, Beijing 102206, China; 2. China National Research Institute of Food & Fermentation Industries Co. Ltd., Beijing 100015, China)
  • Published:2025-06-13

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摘要： 建立一种利用高效液相色谱-荧光检测器同时测定婴幼儿配方奶粉中6 种母乳低聚糖含量的方法。婴幼儿配方奶粉样品首先经酶解和有机溶剂沉淀去除干扰物质，再利用亲水色谱柱进行分离。在最优条件下，6 种母乳低聚糖在其线性范围内线性关系良好，决定系数（R2）均达到0.999以上，加标回收率为95.91%～105.03%，相对标准偏差均小于2%，该方法的检出限为0.003 6～0.006 4 g/100 g，定量限为0.012 0～0.021 4 g/100 g。该方法具有灵敏度高、稳定性好等特点，可以满足婴幼儿配方奶粉中6 种母乳低聚糖的检测需求。

关键词: 母乳低聚糖；婴幼儿配方奶粉；高效液相色谱法；检测；标准

Abstract: A method was developed for the simultaneous determination of six human milk oligosaccharides (HMOs) in infant formula using high performance liquid chromatography-fluorescence detector (HPLC-FLD). The samples were first enzymatically digested and precipitated with organic solvents to remove interfering substances, and then separated using a hydrophilic chromatographic column. Under the optimal conditions, the six HMOs showed good linearity in their respective concentration ranges, with determination coefficients (R2) above 0.999. The recoveries of the spiked samples ranged from 95.91% to 105.03%, with relative standard deviations (RSD) of less than 2%. The limits of detection (LOD) and quantification (LOQ) were in the range of 0.003 6–0.006 4 and 0.012 0–0.021 4 g/100 g, respectively. The developed method is characterized by high sensitivity and good stability, and can meet the requirements of the determination of the six HMOs in infant formula.

Key words: human milk oligosaccharides; infant formula; high performance liquid chromatography; determination; standard

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